Description:
HTT-Q48-PCDNA3.1, CTERM-TEV-FLAG,RDM, 1-3144,
HUMAN (S13A),CODON OPTIMIZED
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Repository
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HD Community Biorepository
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| Quantity |
20 µg |
| Quantitation Method |
Please see our FAQ |
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Alias
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HTT-Q48-PCDNA3.1, CTERM-TEV-FLAG,RDM, 1-3144, HUMAN (S13A),CODON OPTIMIZED
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Family History
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N
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Remarks |
DNA corresponding to the codon
optimized full length human
sequence of the Huntingtin (Htt)
gene containing 48 polyglutamine
repeats translated from a random
codon and containing a S13A
mutation and a C-terminal TEV-FLAG
tag rdm: x/CAG/CAA/x |
| Construct Name |
HTT-Q48-PCDNA3.1, CTERM-TEV-FLAG,RDM, 1-3144, HUMAN (S13A),CODON OPTIMIZED |
| Insert Length |
9564 |
| Vector |
pcDNA3.1 |
| Concentration |
20ug |
| Sequence |
ATGGCTACACTGGAGAAGCTGATGAAAGCATTCGAGGCCCTGAAATCCTTCCAGCAACA
GCAACAGCAACAGCAGCAGCAACAGCAGCAGCAGCAGCAACAGCAACAGCAGCAGCAAC
AGCAACAGCAACAGCAACAGCAACAGCAACAGCAACAGCAACAGCAACAGCAACAGCAA
CAGCAACAGCAACAGCAACCACCTCCCCCTCCACCACCTCCTCCACCACCCCAGCTGCC
ACAGCCACCCCCACAGGCCCAGCCACTGCTGCCACAGCCTCAGCCACCCCCTCCCCCTC
CTCCACCTCCCCCAGGCCCTGCCGTGGCCGAGGAGCCACTGCACAGGCCAAAGAAGGAG
CTGAGCGCCACCAAGAAGGATAGAGTGAACCACTGCCTGACCATCTGCGAGAACATCGT
GGCCCAGTCTGTGAGAAACTCTCCTGAGTTTCAGAAGCTTCTGGGCATCGCCATGGAGC
TGTTCCTGCTGTGCTCCGATGATGCCGAGTCTGACGTGCGGATGGTGGCCGATGAGTGC
CTGAATAAGGTAATCAAGGCCCTGATGGACTCCAACCTGCCCCGGCTGCAGCTGGAGCT
GTACAAGGAGATCAAGAAGAACGGCGCCCCCAGGTCCCTGAGGGCCGCCCTGTGGAGGT
TCGCCGAGCTGGCCCACCTGGTGAGACCTCAGAAGTGCAGACCTTACCTGGTGAACCTG
CTGCCTTGTCTGACACGGACCTCCAAGCGGCCAGAGGAGTCCGTGCAGGAGACACTGGC
CGCCGCCGTGCCTAAGATCATGGCCTCCTTCGGCAATTTCGCCAATGACAATGAGATCA
AGGTGCTGCTGAAGGCCTTCATCGCCAATCTGAAGTCTTCTAGCCCTACAATCCGGAGA
ACAGCCGCCGGCTCTGCCGTGTCCATCTGCCAGCACTCCCGGAGGACCCAGTACTTCTA
TTCCTGGCTGCTGAATGTGCTGCTGGGCCTGCTGGTGCCAGTGGAGGATGAGCACTCTA
CCCTGCTGATCCTGGGCGTGCTGCTGACACTGCGGTACCTGGTGCCACTGCTGCAGCAG
CAGGTGAAGGACACCTCTCTGAAGGGCTCCTTTGGCGTGACAAGAAAGGAGATGGAGGT
GTCCCCCTCTGCCGAGCAGCTGGTGCAGGTGTATGAGCTGACACTGCACCACACCCAGC
ACCAGGACCACAACGTGGTGACAGGCGCCCTGGAGCTGCTGCAGCAGCTGTTCCGGACA
CCACCCCCTGAGCTGCTGCAGACCCTGACAGCCGTGGGCGGCATCGGCCAGCTGACAGC
CGCCAAGGAGGAGTCCGGCGGCCGGTCCAGGTCTGGCTCCATCGTGGAGCTGATCGCCG
GCGGCGGCTCCTCTTGTAGCCCTGTGCTGTCTAGAAAGCAGAAGGGCAAGGTGCTGCTG
GGCGAGGAGGAGGCCCTGGAGGACGACTCTGAGTCCCGGTCCGACGTGAGCTCCTCTGC
CCTGACAGCCTCTGTGAAGGACGAGATCAGCGGCGAGCTGGCCGCCAGCAGCGGCGTGT
CTACCCCAGGCTCCGCCGGCCACGATATCATCACCGAGCAGCCCCGGTCCCAGCACACC
CTGCAGGCCGATTCTGTGGATCTGGCCAGCTGTGATCTGACCAGCTCCGCCACCGACGG
CGACGAGGAGGACATCCTGAGCCACTCCTCCTCCCAGGTGTCTGCCGTGCCATCCGATC
CAGCCATGGATCTGAATGACGGCACCCAGGCCTCTTCCCCCATCAGCGATTCTTCCCAG
ACCACCACAGAGGGCCCAGATTCCGCCGTGACCCCTTCCGATTCTAGCGAGATCGTGCT
GGATGGCACCGACAACCAGTACCTGGGCCTGCAGATCGGCCAGCCACAGGATGAGGACG
AGGAGGCCACCGGCATCCTGCCCGACGAGGCCAGCGAGGCCTTTCGGAACAGCTCCATG
GCCCTGCAGCAGGCCCACCTGCTGAAGAATATGTCTCACTGTAGGCAGCCATCCGATAG
CTCTGTGGATAAGTTCGTGCTGAGGGATGAGGCCACCGAGCCTGGCGACCAGGAGAACA
AGCCCTGTAGAATCAAGGGCGACATCGGCCAGTCTACAGACGATGATAGCGCCCCTCTG
GTGCACTGCGTGCGGCTGCTGTCCGCCTCTTTCCTGCTGACCGGCGGCAAGAACGTGCT
GGTGCCCGATCGGGACGTGAGAGTGTCCGTGAAGGCCCTGGCCCTGTCCTGCGTGGGCG
CCGCCGTGGCCCTGCACCCCGAGAGCTTTTTCTCCAAGCTGTACAAGGTGCCACTGGAT
ACAACAGAGTATCCCGAGGAGCAGTACGTGAGCGACATCCTGAACTATATCGATCACGG
CGACCCCCAGGTGCGGGGCGCCACCGCCATCCTGTGCGGCACCCTGATCTGTTCCATCC
TGTCTAGGTCCCGCTTTCACGTGGGCGATTGGATGGGCACCATCAGAACCCTGACCGGC
AATACCTTTTCTCTGGCCGATTGTATCCCACTGCTGAGAAAGACACTGAAGGATGAGTC
CTCTGTGACATGCAAGCTGGCCTGCACCGCCGTGCGGAATTGCGTGATGTCTCTGTGCT
CCTCCTCCTATTCTGAGCTGGGCCTGCAGCTGATCATCGATGTGCTGACCCTGAGAAAT
TCTTCCTATTGGCTGGTGCGGACAGAGCTGCTGGAGACACTGGCCGAGATCGATTTTCG
CCTGGTGTCTTTCCTGGAGGCCAAGGCCGAGAACCTGCACAGGGGCGCCCACCACTACA
CCGGCCTGCTGAAGCTGCAGGAGAGAGTGCTGAATAACGTGGTAATCCACCTGCTGGGC
GACGAGGATCCAAGAGTGAGACACGTGGCCGCCGCCTCCCTGATCCGCCTGGTGCCTAA
GCTGTTTTACAAGTGTGACCAGGGCCAGGCCGACCCCGTGGTGGCCGTGGCCCGGGATC
AGTCTAGCGTGTATCTGAAGCTGCTGATGCACGAGACACAGCCCCCATCCCACTTTAGC
GTGTCCACCATCACAAGAATCTATCGCGGCTATAACCTGCTGCCATCCATCACAGACGT
GACCATGGAGAATAACCTGTCTCGGGTAATCGCCGCCGTGAGCCACGAGCTGATCACCT
CCACAACCAGGGCCCTGACATTCGGCTGTTGCGAGGCCCTGTGCCTGCTGTCTACCGCC
TTTCCAGTGTGCATCTGGTCCCTGGGCTGGCACTGTGGCGTGCCTCCCCTGTCTGCCTC
CGACGAGTCCAGAAAGTCCTGTACAGTGGGCATGGCCACAATGATCCTGACACTGCTGT
CCTCTGCCTGGTTTCCCCTGGACCTGTCTGCCCACCAGGATGCCCTGATCCTGGCCGGC
AATCTGCTGGCCGCCTCTGCCCCTAAGTCTCTGAGGTCCTCCTGGGCCAGCGAGGAGGA
GGCCAACCCAGCCGCCACCAAGCAGGAGGAGGTGTGGCCTGCCCTGGGCGACAGGGCCC
TGGTGCCTATGGTGGAGCAGCTGTTCTCTCACCTGCTGAAGGTAATCAATATCTGTGCC
CACGTGCTGGATGACGTGGCCCCTGGCCCCGCCATCAAGGCCGCCCTGCCCAGCCTGAC
AAATCCTCCCTCTCTGTCTCCAATCAGACGGAAGGGCAAGGAGAAGGAGCCTGGCGAGC
AGGCCTCTGTGCCTCTGTCCCCCAAGAAGGGCAGCGAGGCCAGCGCCGCCAGCCGCCAG
TCTGATACATCCGGCCCAGTGACAACCAGCAAGTCCAGCTCCCTGGGCTCTTTTTACCA
CCTGCCAAGCTACCTGAAGCTGCACGACGTGCTGAAGGCCACCCACGCCAATTACAAGG
TGACCCTGGACCTGCAGAATAGCACAGAGAAGTTCGGCGGCTTCCTGAGGTCCGCCCTG
GACGTGCTGAGCCAGATCCTGGAGCTGGCCACACTGCAGGATATCGGCAAGTGCGTGGA
GGAGATCCTGGGCTATCTGAAGTCCTGCTTTTCTAGAGAGCCCATGATGGCCACCGTGT
GCGTGCAGCAGCTGCTGAAGACACTGTTCGGCACAAATCTGGCCTCCCAGTTTGACGGC
CTGTCCTCTAATCCAAGCAAGTCCCAGGGCAGGGCCCAGCGGCTGGGCTCCTCCTCTGT
GCGGCCCGGCCTGTATCACTACTGTTTTATGGCCCCATACACCCACTTCACCCAGGCCC
TGGCCGATGCCTCCCTGAGGAACATGGTGCAGGCCGAGCAGGAGAATGATACATCTGGC
TGGTTTGACGTGCTGCAGAAGGTGTCCACCCAGCTGAAGACCAATCTGACATCCGTGAC
AAAGAACCGGGCCGACAAGAATGCCATCCACAACCACATCAGACTGTTTGAGCCACTGG
TAATCAAGGCCCTGAAGCAGTATACCACCACCACCTGCGTGCAGCTGCAGAAGCAGGTG
CTGGACCTGCTGGCCCAGCTGGTGCAGCTGCGCGTGAATTACTGCCTGCTGGATAGCGA
TCAGGTGTTTATCGGCTTCGTGCTGAAGCAGTTCGAGTATATCGAGGTGGGCCAGTTTC
GGGAGTCTGAGGCCATCATCCCCAACATCTTCTTCTTCCTGGTGCTGCTGAGCTATGAG
CGGTATCACAGCAAGCAGATCATCGGCATCCCCAAGATCATCCAGCTGTGCGATGGCAT
CATGGCCAGCGGCAGGAAGGCCGTGACCCACGCCATCCCTGCCCTGCAGCCCATCGTGC
ACGATCTGTTCGTGCTGAGAGGCACCAATAAGGCCGATGCCGGCAAGGAGCTGGAGACC
CAGAAGGAGGTGGTGGTGTCTATGCTGCTGAGACTGATCCAGTATCACCAGGTGCTGGA
GATGTTCATCCTGGTGCTGCAGCAGTGTCACAAGGAGAACGAGGATAAGTGGAAGAGAC
TGTCTAGGCAGATCGCCGATATCATCCTGCCAATGCTGGCCAAGCAGCAGATGCACATC
GATTCCCACGAGGCCCTGGGCGTGCTGAATACACTGTTCGAGATCCTGGCCCCTTCCTC
CCTGAGACCCGTGGACATGCTGCTGAGGAGCATGTTTGTGACACCTAATACAATGGCCT
CTGTGAGCACCGTGCAGCTGTGGATCTCCGGCATCCTGGCCATCCTGCGCGTGCTGATC
TCTCAGAGCACAGAGGATATCGTGCTGTCCCGCATCCAGGAGCTGTCCTTCTCTCCATA
CCTGATCTCTTGTACAGTGATCAACAGGCTGAGAGATGGCGACTCCACATCCACACTGG
AGGAGCACAGCGAGGGCAAGCAGATCAAGAATCTGCCCGAGGAGACATTCAGCCGCTTT
CTGCTGCAGCTGGTGGGCATCCTGCTGGAGGATATCGTGACCAAGCAGCTGAAGGTGGA
GATGTCTGAGCAGCAGCACACCTTCTACTGCCAGGAGCTGGGCACCCTGCTGATGTGCC
TGATCCACATCTTTAAGAGCGGCATGTTTAGACGGATCACCGCCGCCGCCACCAGGCTG
TTCCGGAGCGACGGCTGTGGCGGCTCCTTCTATACCCTGGACAGCCTGAATCTGCGCGC
CCGGTCCATGATCACAACCCACCCAGCCCTGGTGCTGCTGTGGTGTCAGATCCTGCTGC
TGGTGAACCACACCGACTACAGATGGTGGGCCGAGGTGCAGCAGACACCCAAGCGGCAC
TCCCTGTCCTCTACAAAGCTGCTGAGCCCCCAGATGAGCGGCGAGGAGGAGGATTCCGA
TCTGGCCGCCAAGCTGGGCATGTGCAACAGGGAGATCGTGAGAAGGGGCGCCCTGATCC
TGTTTTGCGACTACGTGTGCCAGAATCTGCACGATTCCGAGCACCTGACCTGGCTGATC
GTGAATCACATCCAGGACCTGATCTCTCTGTCTCACGAGCCCCCAGTGCAGGATTTCAT
CTCTGCCGTGCACAGGAATTCTGCCGCCTCCGGCCTGTTTATCCAGGCCATCCAGTCCA
GATGCGAGAATCTGAGCACCCCAACCATGCTGAAGAAGACCCTGCAGTGTCTGGAGGGC
ATCCACCTGAGCCAGAGCGGCGCCGTGCTGACACTGTACGTGGATAGACTGCTGTGCAC
ACCTTTCCGGGTGCTGGCCAGAATGGTGGACATCCTGGCCTGCAGGAGAGTGGAGATGC
TGCTGGCCGCCAATCTGCAGTCCAGCATGGCCCAGCTGCCCATGGAGGAGCTGAATAGA
ATCCAGGAGTACCTGCAGTCCTCTGGCCTGGCCCAGAGACACCAGCGGCTGTATAGCCT
GCTGGATCGGTTCAGGCTGTCCACAATGCAGGACTCCCTGTCCCCAAGCCCACCCGTGA
GCTCCCACCCCCTGGACGGCGATGGCCACGTGTCTCTGGAGACCGTGAGCCCAGACAAG
GATTGGTACGTGCACCTGGTGAAGAGCCAGTGCTGGACCAGATCCGATAGCGCCCTGCT
GGAGGGCGCCGAGCTGGTGAATAGAATCCCAGCCGAGGACATGAACGCCTTTATGATGA
ATTCCGAGTTTAACCTGTCTCTGCTGGCCCCATGTCTGTCTCTGGGCATGTCCGAGATC
TCTGGCGGCCAGAAGTCTGCCCTGTTTGAGGCCGCCCGCGAGGTGACCCTGGCCCGGGT
GAGCGGCACAGTGCAGCAGCTGCCAGCCGTGCACCACGTGTTTCAGCCAGAGCTGCCAG
CCGAGCCCGCCGCCTACTGGTCCAAGCTGAATGACCTGTTCGGCGACGCCGCCCTGTAC
CAGTCTCTGCCAACCCTGGCCAGAGCCCTGGCCCAGTACCTGGTGGTGGTGAGCAAGCT
GCCATCCCACCTGCACCTGCCCCCTGAGAAGGAGAAGGACATCGTGAAGTTCGTGGTGG
CCACCCTGGAGGCCCTGTCTTGGCACCTGATCCACGAGCAGATCCCACTGTCCCTGGAT
CTGCAGGCCGGCCTGGACTGCTGCTGCCTGGCCCTGCAGCTGCCCGGCCTGTGGTCTGT
GGTGTCCTCTACCGAGTTCGTGACCCACGCCTGCAGCCTGATCTATTGCGTGCACTTCA
TCCTGGAGGCCGTGGCCGTGCAGCCAGGCGAGCAGCTGCTGTCCCCAGAGAGGAGAACC
AATACACCCAAGGCCATCTCCGAGGAGGAGGAGGAGGTGGACCCCAACACCCAGAATCC
CAAGTACATCACAGCCGCCTGTGAGATGGTGGCCGAGATGGTGGAGTCCCTGCAGTCTG
TGCTGGCCCTGGGCCACAAGAGGAATAGCGGCGTGCCTGCCTTTCTGACCCCACTGCTG
CGGAACATCATCATCTCCCTGGCCAGGCTGCCTCTGGTGAACAGCTACACCAGAGTGCC
ACCCCTGGTGTGGAAGCTGGGCTGGTCTCCCAAGCCAGGCGGCGATTTCGGCACCGCCT
TCCCCGAGATCCCTGTGGAGTTTCTGCAGGAGAAGGAGGTGTTCAAGGAGTTTATCTAT
AGGATCAATACCCTGGGCTGGACAAGCAGAACCCAGTTCGAGGAGACCTGGGCCACACT
GCTGGGCGTGCTGGTGACCCAGCCACTGGTAATGGAGCAGGAGGAGTCCCCACCTGAGG
AGGATACAGAGAGAACCCAGATCAACGTGCTGGCCGTGCAGGCCATCACCTCCCTGGTG
CTGTCTGCCATGACCGTGCCAGTGGCCGGCAACCCAGCCGTGAGCTGTCTGGAGCAGCA
GCCCAGAAACAAGCCACTGAAGGCCCTGGATACAAGATTCGGCAGAAAGCTGAGCATCA
TCAGAGGCATCGTGGAGCAGGAGATCCAGGCCATGGTGTCTAAGAGGGAGAACATCGCC
ACCCACCACCTGTATCAGGCCTGGGATCCTGTGCCCTCTCTGTCCCCTGCCACCACCGG
CGCCCTGATCTCCCACGAGAAGCTGCTGCTGCAGATCAACCCTGAGAGAGAGCTGGGCT
CTATGAGCTATAAGCTGGGCCAGGTGTCTATCCACAGCGTGTGGCTGGGCAATTCTATC
ACCCCACTGCGGGAGGAGGAGTGGGATGAGGAGGAGGAGGAGGAGGCCGATGCCCCAGC
CCCATCCAGCCCACCCACCTCCCCCGTGAATTCCCGGAAGCACAGAGCCGGCGTGGATA
TCCACAGCTGTTCCCAGTTTCTGCTGGAGCTGTATAGCCGGTGGATCCTGCCTTCTTCC
TCTGCCAGACGCACACCTGCCATCCTGATCAGCGAGGTGGTGCGGAGCCTGCTGGTGGT
GTCCGATCTGTTCACCGAGAGGAATCAGTTCGAGCTGATGTATGTGACACTGACCGAGC
TGCGCAGGGTGCACCCATCTGAGGACGAGATCCTGGCCCAGTATCTGGTGCCCGCCACC
TGTAAGGCCGCCGCCGTGCTGGGCATGGATAAGGCCGTGGCCGAGCCAGTGTCTCGGCT
GCTGGAGAGCACACTGAGATCCAGCCACCTGCCCTCTAGAGTGGGCGCCCTGCACGGCG
TGCTGTACGTGCTGGAGTGCGATCTGCTGGATGACACCGCCAAGCAGCTGATCCCCGTG
ATCTCTGATTACCTGCTGTCCAATCTGAAGGGCATCGCCCACTGTGTGAACATCCACTC
TCAGCAGCACGTGCTGGTAATGTGCGCCACAGCCTTTTACCTGATCGAGAATTATCCTC
TGGATGTGGGCCCAGAGTTCTCCGCCTCTATCATCCAGATGTGCGGCGTGATGCTGAGC
GGCTCCGAGGAGTCTACACCATCCATCATCTATCACTGCGCCCTGAGAGGCCTGGAGAG
ACTGCTGCTGTCTGAGCAGCTGTCTAGACTGGACGCCGAGTCCCTGGTGAAGCTGTCCG
TGGATAGAGTGAACGTGCACTCCCCACACCGGGCCATGGCCGCCCTGGGCCTGATGCTG
ACCTGTATGTACACCGGCAAGGAGAAGGTGAGCCCCGGCAGAACAAGCGACCCAAATCC
CGCCGCCCCAGATTCCGAGAGCGTGATCGTGGCCATGGAGCGCGTGTCCGTGCTGTTCG
ACAGGATCAGAAAGGGCTTCCCATGCGAGGCCCGGGTGGTGGCCAGAATCCTGCCTCAG
TTTCTGGACGACTTCTTTCCTCCTCAGGACATCATGAACAAGGTAATCGGCGAGTTTCT
GTCCAATCAGCAGCCCTACCCCCAGTTTATGGCCACAGTGGTGTACAAGGTGTTTCAGA
CCCTGCACTCCACAGGCCAGAGCTCCATGGTGCGGGATTGGGTAATGCTGAGCCTGTCT
AATTTCACCCAGCGGGCCCCTGTGGCCATGGCCACATGGTCTCTGAGCTGCTTCTTCGT
GTCCGCCTCTACAAGCCCATGGGTGGCCGCCATCCTGCCACACGTGATCTCCCGGATGG
GCAAGCTGGAGCAGGTGGACGTGAATCTGTTCTGTCTGGTGGCCACCGATTTCTACAGG
CACCAGATCGAGGAGGAGCTGGACCGCAGGGCCTTTCAGTCTGTGCTCGAGGTGGTGGC
CGCCCCAGGCAGCCCCTATCACCGCCTGCTGACATGCCTGCGGAATGTGCACAAGGTGA
CCACCTGTGCCGCCGCTGAAAATCTGTACTTCCAGGGAGACTATAAGGATGACGACGAC
AAATGA
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| Cloning Information |
DNA corresponding to the codon optimized full length human sequence of the Huntingtin (Htt) gene containing 48 polyglutamine repeats translated from a random codon and containing a S13A mutation and a C-terminal TEV-FLAG tag rdm: x/CAG/CAA/x |
| Remarks |
DNA corresponding to the codon
optimized full length human
sequence of the Huntingtin (Htt)
gene containing 48 polyglutamine
repeats translated from a random
codon and containing a S13A
mutation and a C-terminal TEV-FLAG
tag rdm: x/CAG/CAA/x |
| DNA corresponding to the codon
optimized full length human
sequence of the Huntingtin (Htt)
gene containing 48 polyglutamine
repeats translated from a random
codon and containing a S13A
mutation and a C-terminal TEV-FLAG
tag rdm: x/CAG/CAA/x |
| Remarks |
DNA corresponding to the codon
optimized full length human
sequence of the Huntingtin (Htt)
gene containing 48 polyglutamine
repeats translated from a random
codon and containing a S13A
mutation and a C-terminal TEV-FLAG
tag rdm: x/CAG/CAA/x |
| PDF |
Vector Map |
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Plasmid Collection Screening and Propagation |
| Supplement |
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