ORDERING CELL CULTURES and DNA SAMPLES
If you are ordering Stem Cells please click HERE for detailed ordering instructions.
- Requirements
- Procedure
- Address
- Shipping Charges
- Shipment of Cell Cultures and DNA samples
- Descriptive Data
REQUIREMENTS
Cell cultures and DNA samples are distributed only to qualified professional persons who are associated with recognized research, medical, educational, or industrial organizations engaged in health-related research or health delivery. There are two requirements for ordering biomaterials from NINDS Human Genetics Resource Center ("NINDS Repository") to ensure compliance with the Office for Protection from Research Risks (OPHR), Department of Health and Human Services (DHHS) regulations for the protection of human subjects (45 CFR Part 46).
- A STATEMENT OF RESEARCH INTENT A description of the research to be done with the cell cultures or DNA samples must be provided to the NINDS Repository.
- An Material Transfer Agreement for Biospecimens (MTA) detailing the terms and conditions of sale must be signed by the principal investigator and the institutional official who can make legal commitments on behalf of the institution.
- Please see Acknowledgement Instructions
Each order placed with the NINDS Repository must be placed electronically using our online catalog system. The information required by the Statement of Research Intent can be submitted electronically during the checkout of the order. The MTA Form (if not already on file) must be submitted by email before an order can be reviewed and approved - this form can be downloaded during the checkout process or may be obtained by contacting Customer Service (see below)
PROCEDURE
All orders for cell cultures or DNA samples must be submitted using the online catalog.
Customers register online and after their registration has been approved, can add items to their
shopping cart from the catalog and checkout.
See first order for more information.
Before requests can be processed, the required information and supporting documentation must be reviewed and approved by the Coriell Order Review Board. If an investigator wishes to order items that do not appear in the catalog they are requested to contact the Coriell Cell Repositories before placing the order (see contact information below).
ADDRESS
All Assurance Forms and any accompanying paperwork should be faxed, mailed, or emailed to:
Coriell Cell Repositories
403 Haddon Avenue
Camden, New Jersey 08103
Telephone: 800-752-3805 in the USA
856-757-4848 from other countries
customerservice@coriell.org
SHIPPING CHARGES
Shipping charges vary depending upon the mode of shipment requested by the purchaser. Domestic shipments are usually made via Federal Express with a guaranteed second day delivery.
SHIPMENT OF CELL CULTURES AND DNA SAMPLES
Cell Cultures
When an order is received, a frozen ampule is usually recovered from liquid nitrogen on the following Thursday. The culture is inspected for growth and contamination and the medium is changed on Friday. The following Monday, the culture is inspected, the medium is removed, and the T25 flask is filled with fresh medium, packed, and shipped. The flask is enclosed within two watertight plastic envelopes within a styrofoam box in a cardboard mailer to prevent leakage, overheating, or freezing during shipment.
Upon receipt, cell cultures should be placed in an incubator at 37C or at the temperature specified on the shipping information sheet for a few hours or overnight without removing any medium to permit recovery from damage which may have occurred during shipment. It is suggested that the recipient store aliquots of cells in early passage in liquid nitrogen to assure that the entire culture is not lost as a result of contamination, artifacts associated with aging, or accidents.
Frozen ampules are not shipped because experience has shown much better success in shipping freshly recovered growing cultures.
Pertinent suggestions to improve the effectiveness of the Repository are appreciated.
Cell Culture Quality Control
The Coriell Cell Repositories has developed and maintains preventative and surveillance procedures to guarantee that the NINDS Human Genetics Resource Center: DNA and Cell Line Repository distributes highly-characterized, viable, and contaminant-free cell cultures.
All cell cultures are grown and frozen in antibiotic-free media to aid in the detection and prevention of contamination. Cultures are tested and found free of mycoplasma, bacteria, and fungi during expansion, at the time of frozen storage, and after recovery of stock for distribution from liquid nitrogen. Recovered cultures are also examined to determine viability and growth potential and to verify species of origin. In addition, gender determination and fingerprinting using highly polymorphic microsatellite markers are performed to avoid cell line misidentification.
DNA Samples
DNA samples are recovered from storage at -70C and shipped on Mondays and Wednesdays at ambient temperature. Recipients should centrifuge the vials prior to opening. Continued storage at -70C is recommended to maintain the integrity of the DNA.
DNA Quality Control
All DNA samples prepared by the Repository are characterized for the following:
- OD260 /OD280 ratio
- concentration
- microsatellite analysis to confirm identity
Feedback from DNA recipients including utilization surveys and publications citing the use of Repository DNA samples have also been reviewed for this purpose.
DESCRIPTIVE DATA
Each shipment includes a packing slip and general information about cell cultures or a listing of DNA concentrations. Specific details for each cell line or DNA sample and pertinent reference citations (if available) are found the Coriell Web Catalog. Search the catalog by using the catalog number.
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