GM23476
iPSC from Fibroblast
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently healthy iPSCs
Apparently Healthy Collection |
| Protocols |
Protocol PDF |
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Biopsy Source
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Skin
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Cell Type
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Stem cell
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Cell Subtype
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Induced pluripotent stem cell
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Transformant
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Reprogrammed (Lentiviral)
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Sample Source
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iPSC from Fibroblast
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Race
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White
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Family Member
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2
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Relation to Proband
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twin sister
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XX[18]
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
35 |
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| Induced Pluripotent Stem Cell |
The frozen cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC and EBs were determined via real-time PCR. Characterization data are included in the Certificate of Analysis. |
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| Remarks |
Induced pluripotent stem cell culture derived from GM04506 Fibroblasts; line HF25; monozygotic twin of GM04505A. Researchers purchasing hiPSCs from the NIGMS Repository are responsible for any limited use label licenses (LULLs) applicable to the cell line purchased. The applicable LULL to this line is WARF. |
| Akter M, Cui H, Sepehrimanesh M, Hosain MA, Ding B, Generation of highly pure motor neurons from human induced pluripotent stem cells STAR protocols3:101223 2022 |
| PubMed ID: 35300000 |
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| Burkhanova U, Harris A, Leir SH, Enhancement of airway epithelial cell differentiation by pulmonary endothelial cell co-culture Stem cell research65:102967 2022 |
| PubMed ID: 36395690 |
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| Sepehrimanesh M, Ding B, Generation and Optimization of Highly Pure Motor Neurons from Human Induced Pluripotent Stem Cells via Lentiviral Delivery of Transcription Factors American journal of physiology Cell physiology65:102967 2020 |
| PubMed ID: 32783653 |
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| Kim E, Wu F, Wu X, Choo HJ, Generation of craniofacial myogenic progenitor cells from human induced pluripotent stem cells for skeletal muscle tissue regeneration Biomaterials248:119995 2019 |
| PubMed ID: 32283390 |
| Passage Frozen |
35 |
| Split Ratio |
1:7 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Knockout DMEM |
| Serum |
20% Knock-out Serum Replacement Not inactivated |
| Substrate |
Gelatin + Feeder Layer |
| Supplement |
Basic Fibroblast Growth Factor 10ng/ml |
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