GM23450

GM23450 iPSC from Fibroblast

Description:

APPARENTLY HEALTHY FETAL TISSUE

Affected:

No

Sex:

Male

Age:

20 FW (At Sampling)

Overview

Repository

NIGMS Human Genetic Cell Repository

Subcollection

Apparently healthy iPSCs
Apparently Healthy Collection

Protocols

Protocol PDF

Biopsy Source

Skin

Cell Type

Stem cell

Cell Subtype

Induced pluripotent stem cell

Transformant

Reprogrammed (Retroviral)

Sample Source

iPSC from Fibroblast

Race

Black/African American

Family Member

Relation to Proband

proband

Confirmation

Karyotypic analysis and Case history

ISCN

46,XY

Species

Homo sapiens

Common Name

Human

Remarks

Induced pluripotent stem cell culture derived from skin fibroblast GM06113; same patient as GM06114. Researchers purchasing hiPSCs from the NIGMS Repository are responsible for any limited use label licenses (LULLs) applicable to the cell line purchased. The applicable LULL to this line is WARF.

Characterizations

Passage Frozen

Induced Pluripotent Stem Cell

The cell line submitted to the Repository frozen was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation and directed differentiation toward cardiac neuronal and pancreatic lineages. Steady-state mRNA expression patterns of undifferentiated iPSC EB and differentiated iPSC were determined via real-time PCR. The line was evaluated for in vivo pluripotency via teratoma formation assay. Characterization data are included in the Certificate of Analysis.

Phenotypic Data

Remarks

Culture Protocols

Passage Frozen

Split Ratio

1:8

Temperature

37 C

Percent CO2

Percent O2

AMBIENT

Medium

Ham's F12 Medium/Dulbecco Modified Eagles Medium, 1:1 mixture with 2mM L-glutamine or equivalent

Serum

20% Knock-out Serum Replacement

Substrate

Gelatin + Feeder Layer

Supplement

Pricing

Commercial/For-profit:

$1,789.00USD

Academic/Non-profit/Government:

$1,110.00USD