GM06111
Fibroblast from Skin, Unspecified
Description:
APPARENTLY HEALTHY FETAL TISSUE
Repository
|
NIGMS Human Genetic Cell Repository
|
Subcollection |
Apparently Healthy Collection |
Biopsy Source
|
Unspecified
|
Cell Type
|
Fibroblast
|
Tissue Type
|
Skin
|
Transformant
|
Untransformed
|
Sample Source
|
Fibroblast from Skin, Unspecified
|
Race
|
Black/African American
|
Family Member
|
1
|
Relation to Proband
|
proband
|
Confirmation
|
Karyotypic analysis and Case history
|
ISCN
|
46,XX.arr 7q11.23(73728758-73964249)x1,8p23.1(7284737-7791790)x1
|
Species
|
Homo sapiens
|
Common Name
|
Human
|
Remarks
|
|
PDL at Freeze |
7.05 |
Passage Frozen |
7 |
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
|
Remarks |
Skin biopsy; same patient as GM06112; 46,XX; 6% of cells show random chromosome loss |
Tang Z, Berlin DS, Toji L, Toruner GA, Beiswanger C, Kulkarni S, Martin CL, Emanuel BS, Christman M, Gerry NP, A dynamic database of microarray-characterized cell lines with various cytogenetic and genomic backgrounds G3 (Bethesda, Md)3:1143-9 2013 |
PubMed ID: 23665875 |
|
Karlsson C, Paulsson Y, Age related induction of platelet-derived growth factor A-chain mRNA in normal human fibroblasts. J Cell Physiol158:256-62 1994 |
PubMed ID: 8106562 |
|
Ethier MF, Hickler RB, Dobson JG Jr, Aging increases adenosine and inosine release by human fibroblast cultures. Mech Ageing Dev50:159-68 1989 |
PubMed ID: 2601414 |
Passage Frozen |
7 |
Split Ratio |
1:3 |
Temperature |
37 C |
Percent CO2 |
5% |
Percent O2 |
AMBIENT |
Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
Serum |
15% fetal bovine serum Not inactivated |
Substrate |
None specified |
Supplement |
- |
|
|