GM03234
Fibroblast from Skin, Arm
Description:
APPARENTLY HEALTHY INDIVIDUAL
Repository
|
NIGMS Human Genetic Cell Repository
|
Subcollection |
Apparently Healthy Collection |
Biopsy Source
|
Arm
|
Cell Type
|
Fibroblast
|
Tissue Type
|
Skin
|
Transformant
|
Untransformed
|
Sample Source
|
Fibroblast from Skin, Arm
|
Race
|
American Indian/Alaska Native
|
Ethnicity
|
Pima Indian
|
Relation to Proband
|
proband
|
Confirmation
|
Karyotypic analysis and Case history
|
Species
|
Homo sapiens
|
Common Name
|
Human
|
PDL at Freeze |
5.68 |
Passage Frozen |
6 |
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
|
Venkatesh A, McKenty T, Ali S, Sonntag D, Ravipaty S, Cui Y, Slate D, Lin Q, Christiansen A, Jacobson S, Kach J, Lim KH, Srinivasan V, Zinshteyn B, Aznarez I, Huryn LA, Li Z, Hufnagel RB, Liau G, Anderson K, Hoger J, Antisense Oligonucleotide STK-002 Increases OPA1 in Retina and Improves Mitochondrial Function in Autosomal Dominant Optic Atrophy Cells Nucleic acid therapeutics: 2024 |
PubMed ID: 39264859 |
|
Fleischer JG, Schulte R, Tsai HH, Tyagi S, Ibarra A, Shokhirev MN, Huang L, Hetzer MW, Navlakha S, Predicting age from the transcriptome of human dermal fibroblasts Genome Biology19: 2018 |
PubMed ID: 30567591 |
|
Goldman D, Giri PR, O'Brien SJ, A molecular phylogeny of the hominoid primates as indicated by two- dimensional protein electrophoresis. Proc Natl Acad Sci U S A84:3307-11 1987 |
PubMed ID: 3106965 |
|
Goldman D, Goldin LR, Rathnagiri P, O'Brien SJ, Egeland JA, Merril CR, Twenty-seven protein polymorphisms by two-dimensional electrophoresis of serum, erythrocytes, and fibroblasts in two pedigrees. Am J Hum Genet37:898-911 1985 |
PubMed ID: 3863481 |
|
Howard BV, Fields RM, Mott DM, Savage PJ, Nagulesparan M, Bennett PH, Diabetes and cell growth--lack of differences in growth characteristics of fibroblasts from diabetic and nondiabetic Pima Indians. Diabetes29:119-24 1980 |
PubMed ID: 7353728 |
Passage Frozen |
6 |
Split Ratio |
1:2 |
Temperature |
37 C |
Percent CO2 |
5% |
Percent O2 |
AMBIENT |
Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
Serum |
15% fetal bovine serum Not inactivated |
Supplement |
- |
|
|