AG27607

AG27607 iPSC from Fibroblast

Description:

ALZHEIMER DISEASE; AD
APOLIPOPROTEIN E; APOE

Affected:

Yes

Sex:

Female

Age:

69 YR (At Sampling)

Overview

Repository

NIA Aging Cell Culture Repository

Subcollection

Alzheimer's Disease

Protocols

Protocol PDF

Biopsy Source

Skin

Cell Type

Stem cell

Cell Subtype

Induced pluripotent stem cell

Transformant

Reprogrammed (Retroviral)

Sample Source

iPSC from Fibroblast

Race

White

Family Member

Family History

Relation to Proband

proband

Confirmation

Clinical summary/Case history

ISCN

46,XX[20]

Species

Homo sapiens

Common Name

Human

Remarks

Clinically affected; hiPSC line was derived from externally submitted iPSC line, in which a distribution stock was made. Please see Publication Meyer et al.,2019; Parental fibroblast is AG21158. Researchers purchasing hiPSCs from the NIA Repository are responsible for any limited use label licenses (LULLs) applicable to the cell line purchased. The applicable LULL to this line is iPS Academia Japan, Inc.

Characterizations

Passage Frozen

Induced Pluripotent Stem Cell

The frozen cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC and EBs were determined via real-time PCR. Characterization data are included in the Certificate of Analysis.

Phenotypic Data

Remarks

Publications

Meyer K, Feldman HM, Lu T, Drake D, Lim ET, Ling KH, Bishop NA, Pan Y, Seo J, Lin YT, Su SC, Church GM, Tsai LH, Yankner BA, REST and Neural Gene Network Dysregulation in iPSC Models of Alzheimer's Disease Cell reports26:1112-1127.e9 2018

PubMed ID: 30699343