AG22339
LCL from B-Lymphocyte
Description:
PROSTATE CANCER
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Repository
|
NIA Aging Cell Culture Repository
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| Subcollection |
Prostate Cancer |
|
Biopsy Source
|
Peripheral vein
|
|
Cell Type
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B-Lymphocyte
|
|
Tissue Type
|
Blood
|
|
Transformant
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Epstein-Barr Virus
|
|
Sample Source
|
LCL from B-Lymphocyte
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|
Race
|
White
|
|
Family Member
|
1
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Clinical summary/Case history
|
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ISCN
|
46,XY
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|
Species
|
Homo sapiens
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|
Common Name
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Human
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|
Remarks
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|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin confirmed by LINE assay |
| |
| Remarks |
The donor had been diagnosed with prostate cancer at age 70. The culture was initiated on 4/23/08 by transformation of lymphocytes with Epstein Barr virus. The culture grows in suspension and the cell morphology is spherical. |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
|
|