AG22161
Fibroblast from Skin, Toe
Description:
WOUND HEALING DISORDER
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Non-Healing Wound Collection |
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Biopsy Source
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Toe
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Toe
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Race
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White
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Family Member
|
1
|
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Family History
|
N
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Relation to Proband
|
proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Senescence |
21 |
| PDL at Freeze |
4.72 |
| Passage Frozen |
3 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin confirmed by LINE assay |
| |
| Remarks |
Culture established from explants of 2cm of skin punched out of an ulcer on the plantar surface of the left great toe; hypertension; myocardial infarction eleven years ago treated with two stents implanted during angioplasty; diabetic; melanoma removed from scalp with skin flap; ulcer had no pus, erythema or tenderness; subject denies trauma or pain and can ambulate; xray shows no acute disease in the left foot |
| PDL at Senescence |
21 |
| Cumulative PDL at Freeze |
4.72 |
| Passage Frozen |
3 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Supplement |
- |
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