AG13074
Fibroblast from Skin, Skin
Description:
ANEUPLOID CHROMOSOME NUMBER - TRISOMY 18
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Chromosome Abnormalities |
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Biopsy Source
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Skin
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Skin
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Race
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White
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XX/47,XX,+18
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
3 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Culture was initiated from explants of skin obtained post-mortem from a 19 week gestational age fetus. The cell morphology is fibroblast-like. The culture is a mosaic with karyotype: 46,XX/47,XX,+18; 30%/70%. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Stamoulis G, Garieri M, Makrythanasis P, Letourneau A, Guipponi M, Panousis N, Sloan-Béna F, Falconnet E, Ribaux P, Borel C, Santoni F, Antonarakis SE, Single cell transcriptome in aneuploidies reveals mechanisms of gene dosage imbalance Nature communications10:4495 2019 |
| PubMed ID: 31582743 |
| Passage Frozen |
3 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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