AG12614
Fibroblast from Fetal, Unspecified
Description:
ANEUPLOID CHROMOSOME NUMBER - TRISOMY 18
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Chromosome Abnormalities |
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Biopsy Source
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Unspecified
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Cell Type
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Fibroblast
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Tissue Type
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Fetal
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Fetal, Unspecified
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Race
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White
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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47,XX,+18
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
6 |
| Passage Frozen |
3 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
The culture was initiated using explants of minced fetal tissue obtained post-mortem. The age of the fetus at the time of the biopsy is unknown. The cell morphology is fibroblast-like. The karyotype is 47,XX,+18. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Kinde I, Papadopoulos N, Kinzler KW, Vogelstein B, FAST-SeqS: a simple and efficient method for the detection of aneuploidy by massively parallel sequencing PloS one7:e41162 2012 |
| PubMed ID: 22815955 |
| Cumulative PDL at Freeze |
6 |
| Passage Frozen |
3 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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