AG10028
Fibroblast from Skin, Neck
Description:
XERODERMA PIGMENTOSUM: COMPLEMENTATION GROUP UNDETERMINED
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Heritable Diseases |
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Biopsy Source
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Neck
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Neck
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Race
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White
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Ethnicity
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SOUTH AMERICAN
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
4 |
| Passage Frozen |
4 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
The Chilean donor is clinically affected with features of multiple lesions, extensive freckling, and scarring of the left cornea due to exposure keratitis. She has facial tumors of the left and right cheeks. The right cheek tumor was excised and found to be malignant melanoma. She had no neurological involvement. She has two normal sibs. The biopsy was taken ante-mortem on 5/10/88 from an uninvolved area on the donor's neck. The culture was initiated using explants of minced skin tissue. The cell morphology is fibroblast-like. Culture is frozen at PDL 4. |
| Cumulative PDL at Freeze |
4 |
| Passage Frozen |
4 |
| Split Ratio |
1:2 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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