AG07443
Fibroblast from Skin, Skin
Description:
ANEUPLOID CHROMOSOME NUMBER - NON-TRISOMIC
|
Repository
|
NIA Aging Cell Culture Repository
|
| Subcollection |
Chromosome Abnormalities |
|
Biopsy Source
|
Skin
|
|
Cell Type
|
Fibroblast
|
|
Tissue Type
|
Skin
|
|
Transformant
|
Untransformed
|
|
Sample Source
|
Fibroblast from Skin, Skin
|
|
Race
|
White
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Clinical summary/Case history
|
|
ISCN
|
45,X
|
|
Species
|
Homo sapiens
|
|
Common Name
|
Human
|
|
Remarks
|
|
| PDL at Freeze |
6 |
| Passage Frozen |
2 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
| |
| Remarks |
The donor was diagnosed as Turner syndrome based on karyotype analysis of blood and skin prior to submission to the Repository. The culture was initiated on 2/25/84 using explants of minced skin tissue obtained postmortem from this fetus of approximately 20 weeks gestational age. The cell morphology is fibroblast-like. The karyotype is 45,X. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Cumulative PDL at Freeze |
6 |
| Passage Frozen |
2 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
|
|