AG04765
Mesothelial from Lung
Description:
RATTUS
ORDER: RODENTIA
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals |
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Biopsy Source
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Lung
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Cell Type
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Mesothelial
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Tissue Type
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Lung
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Transformant
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Untransformed
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Sample Source
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Mesothelial from Lung
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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41,X/41,X,11q+
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Species
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Rattus norvegicus
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Common Name
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brown or Norway rat
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Remarks
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| PDL at Freeze |
33 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Genbank |
COX1 |
| Remarks |
Culture derived from a Fisher 344 rat of Charles River strain. The culture was initiated on 2/8/78 by trypsin digestion of an intact left lung lobe. The released cells were plated on cover-slip fragments and fragments with only epithelial cells were passaged. Cell morphology is epithelial-like. The karyotype is near diploid: 41,X/41,X,11q+; 60%/40%. The culture may be immortal. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Cumulative PDL at Freeze |
33 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
20% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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