AG03962
Fibroblast from Skin, Arm
Description:
XERODERMA PIGMENTOSUM, COMPLEMENTATION GROUP C; XPC
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Heritable Diseases |
| Class |
Repair Defective and Chromosomal Instability Syndromes |
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Biopsy Source
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Arm
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Arm
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Race
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White
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Ethnicity
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EGYPTIAN
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XX
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
5 |
| Passage Frozen |
4 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Remarks |
The Egyptian donor showed mild skin features of XP, mainly depigmentation of face and hands. She had no neurological involvement. The skin biopsy was taken ante-mortem on 3/19/80. The culture was initiated using explants of minced skin tissue. The cell morphology is fibroblast-like. Karyotype is 46,XX; normal diploid female. Complementation group assignment and DNA repair deficiency have been verified for this culture (XP11CA). The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Needleman SW, Yuasa Y, Srivastava S, Aaronson SA, Normal cells of patients with high cancer risk syndromes lack transforming activity in the NIH/3T3 transfection assay. Science222:173-5 1983 |
| PubMed ID: 6623066 |
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| Cleaver JE, Zelle B, Hashem N, El-Hefnawi MH, German J, Xeroderma pigmentosum patients from Egypt: II. Preliminary correlations of epidemiology, clinical symptoms and molecular biology. J Invest Dermatol77:96-101 1981 |
| PubMed ID: 7252263 |
| Cumulative PDL at Freeze |
5 |
| Passage Frozen |
4 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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