I91S-05
Fibroblast from Skin, Skin
Description:
IMR-91 - NORMAL HUMAN FETAL FIBROBLAST
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
IMR90/IMR91 Cell Cultures
Specially Characterized Fibroblasts |
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Biopsy Source
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Skin
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Skin
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Race
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White
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
10 |
| Passage Frozen |
10 |
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| Remarks |
The IMR-91 skin-derived line was initiated on 2/24/76 from explants of minced skin tissue from an approximately 12 week fetus. The cell morphology is fibroblast-like. The karyotype is 46,XY; normal diploid male. The HLA type is A2, A11, B18 and B5. G6PD is isoenzyme type B and 6PGD is isoenzyme type A. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Nichols WW, Cristofalo VJ, Toji LH, Greene AE, Aronson MM, Dwight S, Charpentier R, Hoffman E, Characterization of a new human diploid cell line--IMR-91. In Vitro19:797-804 1983 |
| PubMed ID: 6629383 |
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| Brown WT, Ford JP, Gershey EL, Variation of DNA repair capacity in progenia cells unrelated to growth conditions. Biochem Biophys Res Commun97:347-53 1980 |
| PubMed ID: 7458939 |
| Cumulative PDL at Freeze |
20 |
| Passage Frozen |
10 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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