GM29806
iPSC from Fibroblast
Description:
INDUCIBLE CRISPRA-I3M (MICROGLIA) WTC11 IPSC LINE
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently healthy iPSCs
Apparently Healthy Collection |
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License Required
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For commercial institutions interested in purchasing this iPSC line, additional licensing may be required. Please reach out to our customer service team at customerservice@coriell.org for more information.
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| Protocols |
Protocol PDF |
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Biopsy Source
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Skin
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Cell Type
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Stem cell
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Cell Subtype
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Induced pluripotent stem cell
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Transformant
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Reprogrammed (Episomal)
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Sample Source
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iPSC from Fibroblast
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Race
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Asian
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Ethnicity
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Not Hispanic/Latino
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Ethnicity
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Japanese
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Country of Origin
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USA
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Family Member
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1
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Family History
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N
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Relation to Proband
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proband
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Confirmation
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Karyotypic analysis before cell line submission to CCR
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ISCN
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46,XY[19]
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
96 |
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| Induced Pluripotent Stem Cell |
The gene-edited cell line was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC and EBs were determined via real-time PCR. Characterization data are included in the Certificate of Analysis. |
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| Remarks |
This cell line is a gene edited iPSC line, (WTC11 - Inducible CRISPRa-i3M (Microglia)), generated using TALEN-mediated insertion into safe harbor locus. The parental cell source is described in PMID: 24509632. Induced pluripotent stem cell line WTC11 derived from adult skin (leg) fibroblasts; subject clinically normal; wild-type; FISH test: 46, XY. Also available is a collection of fluorescently tagged hiPSC lines from the same subject, using CRISPR/Cas9 technology (for more information, see the Allen Cell Collection at Coriell: https://catalog.coriell.org/1/AllenCellCollection). No secondary distribution of this line is permitted. Researchers purchasing hiPSCs from the NIGMS Repository are responsible for any limited use label licenses (LULLs) applicable to the cell line purchased. The applicable LULL to this line is iPS Academia, Japan Inc.. |
| Miyaoka Y, Chan AH, Judge LM, Yoo J, Huang M, Nguyen TD, Lizarraga PP, So PL, Conklin BR, Isolation of single-base genome-edited human iPS cells without antibiotic selection Nature methods11:291-3 2013 |
| PubMed ID: 24509632 |
| Passage Frozen |
96 |
| Split Ratio |
1:10 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
mTeSR1 |
| Serum |
0% none |
| Substrate |
Matrigel |
| Supplement |
- |
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