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GM28907
iPSC
from
Fibroblast
Description:
APPARENTLY HEALTHY INDIVIDUAL
Affected:
No
Sex:
Female
Age:
16
YR
(At Sampling)
Sample Description
Overview
Characterizations
Phenotypic Data
Culture Protocols
Overview
Repository
NIGMS Human Genetic Cell Repository
Subcollection
Apparently healthy iPSCs
Apparently Healthy Collection
Protocols
Protocol PDF
Biopsy Source
Skin
Cell Type
Stem cell
Cell Subtype
Induced pluripotent stem cell
Transformant
Reprogrammed (Sendai)
Sample Source
iPSC from Fibroblast
Race
Other
Ethnicity
White mother and Japanese father
Family Member
1
Relation to Proband
proband
Confirmation
Clinical summary/Case history
ISCN
46,XX[20]
Species
Homo
sapiens
Common Name
Human
Remarks
Reprogrammed from parental line GM07532 (fibro); same subject as GM07533 (lymph). The Researchers purchasing hiPSCs from the NIGMS Repository are responsible for any limited use label licenses (LULLs) applicable to the cell line purchased. The applicable LULL to this line is
Sendai-CytoTune
.
Characterizations
Passage Frozen
13
Induced Pluripotent Stem Cell
The parental cell line was recovered reprogrammed to an induced pluripotent stem cell line and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated iPSC and EBs were determined via real-time PCR. Characterization data are included in the
Certificate of Analysis.
Phenotypic Data
Remarks
Reprogrammed from parental line GM07532 (fibro); same subject as GM07533 (lymph). The Researchers purchasing hiPSCs from the NIGMS Repository are responsible for any limited use label licenses (LULLs) applicable to the cell line purchased. The applicable LULL to this line is
Sendai-CytoTune
.
Culture Protocols
Passage Frozen
13
Split Ratio
1:6
Temperature
37 C
Percent CO2
5%
Percent O2
AMBIENT
Medium
mTeSR1
Serum
none
Substrate
Matrigel
Supplement
-
Pricing
Commercial/For-profit:
$1,789.00
USD
Academic/Non-profit/Government:
$1,110.00
USD
Add to Cart
How to Order
Ordering Instructions
MTA / Assurance Form
Statement of Research Intent Form
Related Products
Same Subject
NA07532 - DNA
GM07532 - Fibroblast
GM07533 - B-Lymphocyte
Same Family
1057
Miscellaneous
DNA on Demand
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