Description:
TRANSGENIC MOUSE ES
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals
Stem Cell Collection |
| Protocols |
Protocol PDF |
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Cell Type
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Stem cell
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Tissue Type
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Embryonic
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Transformant
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pZhcSfi
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ISCN
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39,X[4]/40,XY,add(1)(H6),add(8)(A1)[2]/40,XY,add(1)(H6)[1]/40,XY[17]
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Species
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Mus musculus
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Common Name
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house mouse
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Remarks
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| Passage Frozen |
4 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| mouse embryonic stem cell |
The cell line submitted to the Repository frozen was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation. Steady-state mRNA expression patterns of undifferentiated mES and EB were determined via real-time PCR. Characterization data are included in the Certificate of Analysis. |
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| Nishiyama A, Xin L, Sharov AA, Thomas M, Mowrer G, Meyers E, Piao Y, Mehta S, Yee S, Nakatake Y, Stagg C, Sharova L, Correa-Cerro LS, Bassey U, Hoang H, Kim E, Tapnio R, Qian Y, Dudekula D, Zalzman M, Li M, Falco G, Yang HT, Lee SL, Monti M, Stanghellini I, Islam MN, Nagaraja R, Goldberg I, Wang W, Longo DL, Schlessinger D, Ko MS, Uncovering early response of gene regulatory networks in ESCs by systematic induction of transcription factors Cell stem cell5:420-33 2009 |
| PubMed ID: 19796622 |
| Passage Frozen |
4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum |
| Substrate |
Gelatin + Feeder Layer |
| Supplement |
- |
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