GM16245
Somatic cell hybrid
Description:
CHROMOSOME 17 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
ADDITIONAL DELETION/DERIVATIVE CHROMOSOME 7 SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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ISCN
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Human/hamster somatic cell hybrid retaining a human der(7)t(7;17)(q34;q25.1) in 100% of cells and a 4, 13, 16, 20, 22, & Y
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Clone of GM15776 produced by the fusion of human cells with karyotype 46,XY,t(7;17) (q34;q25.1) with TK- hamster A3 cells; grown in medium containing HAT; retains human der(7)t(7;17)(q34;q25.1) in 100% of cells and other human chromosomes. |
| Split Ratio |
1:6 |
| Temperature |
37 C |
| Percent CO2 |
8% |
| Medium |
Dulbecco Modified Eagles Medium (high glucose) with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Aminopterin 4 x 10-7M |
| Supplement |
Hypoxanthine 1 x 10-4M |
| Supplement |
Thymidine 1.6 x 10-5M |
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