GM14969
Somatic cell hybrid
Description:
CHROMOSOME 16 REGIONAL MAPPING PANEL SOMATIC CELL HYBRIDS
ADDITIONAL DELETION/DERIVATIVE CHROMOSOME 6 SOMATIC CELL HYBRIDS
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Hybrids |
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Cell Type
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Somatic cell hybrid
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Transformant
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Untransformed
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Confirmation
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Clinical summary/Case history
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ISCN
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Human/mouse somatic cell hybrid retaining a human der(16)t(6;16)(p21.3;p13.11) in 96% of cells and a human #13
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Line CY185; produced by the fusion of human fibroblast line ZEH, 46,XX,t(6;16) (p21.3;p13.1), with mouse A9 cells; culture grown in medium containing adenine and azaserine; retains human der(16)t(6;16) (p21.3;p13.11) in 96% of cells and 13 (24%) |
| Split Ratio |
1:7 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F12 with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Adenine 5 x 10-5mM |
| Supplement |
Azaserine 5 x 10-5mM |
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