GM24109
LCL from B-Lymphocyte
Description:
PERSONAL GENOME PROJECT
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Apparently Healthy Collection
PIGI Consented Sample
Whole-Genome Sequence |
|
Biopsy Source
|
Peripheral vein
|
|
Cell Type
|
B-Lymphocyte
|
|
Tissue Type
|
Blood
|
|
Transformant
|
Epstein-Barr Virus
|
|
Sample Source
|
LCL from B-Lymphocyte
|
|
Race
|
White
|
|
Ethnicity
|
Canadian, Dutch (Netherlands), UK, French, Irish
|
|
Country of Origin
|
USA
|
|
Family History
|
N
|
|
Relation to Proband
|
proband
|
|
Species
|
Homo sapiens
|
|
Common Name
|
Human
|
|
Remarks
|
|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by LINE assay |
| |
| Remarks |
Participant (hu5FA322) in the Personal Genome Project: http://www.personalgenomes.org
Atypical porphyria with symptom onset at age range 10-19 years; colon polyps; uterine fibroids; cervical cancer; uterine fibroids; fibrocystic breast disease; hypothyroidism; gout; iron deficiency anemia; essential tremor; epilepsy; cluster headaches; migraine without aura; carpal tunnel syndrome; age-related cataract; myopia; hyperopia; floaters; astigmatism; age-related hearing loss; tinnitus; pulmonary embolism; atrial fibrillation; cardiac arrhythmia; aortic aneurysm; deep vein thrombosis; varicose veins; hemorrhoids; dental cavities; gastroesophageal reflux disease; appendicitis; hiatal hernia; diverticulosis; gallstones; fibrous liver/gall bladder; kidney stones; urinary tract infections; skin tags; hair loss; café au lait spots; lupus; rheumatoid arthritis; osteoarthritis; spinal stenosis; sciatica; bone spurs; osteoporosis; scoliosis; poor detoxification; mitochondrial myopathy; peripheral neuropathy; chronic sinusitis; ulcerative colitis; irritable bowel syndrome; procedures include hysterectomy due to pre-cancerous uterine cells. Whole genome sequence data is available on the PGP website. |
| Smullen M, Olson MN, Reichert JM, Dawes P, Murray LF, Baer CE, Wang Q, Readhead B, Church GM, Lim ET, Chan Y, Reliable multiplex generation of pooled induced pluripotent stem cells Cell reports methods3:100570 2022 |
| PubMed ID: 37751688 |
| |
| Mao Q, Ciotlos S, Zhang RY, Ball MP, Chin R, Carnevali P, Barua N, Nguyen S, Agarwal MR, Clegg T, Connelly A, Vandewege W, Zaranek AW, Estep PW, Church GM, Drmanac R, Peters BA., The whole genome sequences and experimentally phased haplotypes of over 100 personal genomes. Gigascience.5(1):42 2016 |
| PubMed ID: 27724973 |
| |
| Church GM, The personal genome project. Mol Syst Biol.1, 2005.0030:42 2005 |
| PubMed ID: 16729065 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
|
|