GM24085
LCL from B-Lymphocyte
Description:
PERSONAL GENOME PROJECT
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently Healthy Collection
PIGI Consented Sample
Whole-Genome Sequence |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Sample Source
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LCL from B-Lymphocyte
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Race
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White
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Family History
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N
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Relation to Proband
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proband
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by LINE assay |
| |
| Remarks |
Participant (huED0F40) in the Personal Genome Project: http://www.personalgenomes.org Osteoarthritis; bone spurs; Scheuermann’s kyphosis; Schmorl’s nodes (thoracic vertebrae); flat feet; joint hypermobility: thumbs, wrists and hips; myopia (nearsightedness); astigmatism; sensorineural hearing loss (left ear); choroidal nevus (left eye); minor retinal lattice degeneration; hypertension; mitral valve prolapsed; hypertrophic cardiomyopathy; cardiac arrhythmia; myxomatous degeneration of mitral valves; social anxiety; depression; mood swings; insomnia; dental caries; canker sores; chronic flatulence; genital molluscum contagiosum; dermatitis; petechia (trunk/thighs); premature ejaculation; narrow misshaped heels; vaulted U-shaped palate micrognathism; low serum HDL, vitamin D3 deficiency; lichen planus; skin tags; hyperhidrosis; acne; café au lait spots; allergic rhinitis; myopia; astigmatism; allergies include: Abyssinian cat dander, grass pollen-Timothy, standard; tree pollen; ragweed and soil mold; procedures include: radial keratotomy; medications include: gabapentin; allergy antigen injections; Avapro; hydralazine-hydrochlorothiazide; losartan; family history: father had mitral valve prolapsed ; enlarged aortic stem and early onset spinal arthritis; several uncles had hip dysplasia; multiple hiatal and inguinal hernias and chronic pain. Whole genome sequence data is available on the PGP website. |
| Smullen M, Olson MN, Reichert JM, Dawes P, Murray LF, Baer CE, Wang Q, Readhead B, Church GM, Lim ET, Chan Y, Reliable multiplex generation of pooled induced pluripotent stem cells Cell reports methods3:100570 2022 |
| PubMed ID: 37751688 |
| |
| Mao Q, Ciotlos S, Zhang RY, Ball MP, Chin R, Carnevali P, Barua N, Nguyen S, Agarwal MR, Clegg T, Connelly A, Vandewege W, Zaranek AW, Estep PW, Church GM, Drmanac R, Peters BA., The whole genome sequences and experimentally phased haplotypes of over 100 personal genomes. Gigascience.5(1):42 2016 |
| PubMed ID: 27724973 |
| |
| Church GM, The personal genome project. Mol Syst Biol.1, 2005.0030:42 2005 |
| PubMed ID: 16729065 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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