GM20935
LCL from B-Lymphocyte
Description:
HEMOGLOBIN F, HEREDITARY PERSISTENCE OF
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Heritable Diseases |
| Class |
Mutations of the Hemoglobin Loci |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Sample Source
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LCL from B-Lymphocyte
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Race
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Black/African American
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Ethnicity
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AFRICAN-AMERICAN
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Family Member
|
1
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Relation to Proband
|
proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin confirmed by LINE assay |
| |
| Remarks |
Clinically healthy; hereditary persistence of fetal hemoglobin; childhood asthma; hypothyroidism; premature ovarian failure; hemoglobin electrophoresis results: HbA = 68.5%, HbA2 = 2.1%, HbF = 29.4%, HbS = 0%; hemoglobin = 13.3 g/dl; MCV = 79.8; mother of GM20936 and GM20937.
|
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Supplement |
- |
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