GM16727
LCL from B-Lymphocyte
Description:
LYMPHOBLASTIC LEUKEMIA, ACUTE, WITH LYMPHOMATOUS FEATURES; LALL
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Heritable Diseases |
| Class |
Heritable Cancer Syndromes and other Cancers |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Herpes Simplex Virus Type II
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Sample Source
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LCL from B-Lymphocyte
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Race
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Black/African American
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Karyotypic analysis after cell line submission to CCR
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ISCN
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46~49,XY,t(8;9)(q13;p24),del(13)(q12q14),add(22)(q13.2),+markers[cp10]
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Cytogenetics |
Chromosome 13: DELETION Aneuploid Segment (-)13q12>13q14 |
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Chromosome 9: TRANSLOCATION Breakpoint 9p24 t(8;9)9p24 |
| Remarks |
Line KLC-HSV2; this sub-cell line was derived by infecting line KLC (GM16726) with herpes simplex virus type II (HSV2). This is the first human lymphoid cell line to show persistent in vitro HSV-2 infection extending over several years [Kao, Y.S., Sundin, D.R., and Gebhardt, B.M. Persistent infection of a lymphoma cell line by Herpes Simplex Virus, Am J Hematol 62:93-98 (1999)]. The NIGMS Repository does not guarantee HSV-2 expression in this cell line. |
| Kao YS, Sundin DR, Gebhardt BM, Persistent infection of a lymphoma cell line by herpes simplex virus. Am J Hematol62(2):93-8 1999 |
| PubMed ID: 10509003 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
20% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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