GM11431
Amniotic fluid-derived cell line from Amniotic fluid
Description:
FRAGILE X MENTAL RETARDATION SYNDROME
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Heritable Diseases
Chromosome Abnormalities |
| Class |
Disorders with Trinucleotide Expansions |
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Biopsy Source
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Amniotic fluid
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Cell Type
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Amniotic fluid-derived cell line
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Transformant
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pSVori plasmid
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Sample Source
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Amniotic fluid-derived cell line from Amniotic fluid
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
pSVori-transformed C5807 amniocyte cells; obligate carrier for fra(X); precrisis clone #1 expresses the fra(X) in 9% of cells; line is pseudodiploid, polyploid, & has rearrangements |
| Jenkins LS, Gruenert DC, Jenkins EC, Schonberg SA, Transformation and establishment of fragile X cell lines from amniocytes. Am J Med Genet38:416-7 1991 |
| PubMed ID: 1850193 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Amniotic Fluid Culture Medium |
| Serum |
none Heat Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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