GM07427
LCL from B-Lymphocyte
Description:
WILMS TUMOR 1; WT1
|
Repository
|
NIGMS Human Genetic Cell Repository
|
| Subcollection |
Heritable Diseases
dbGaP |
| Class |
Heritable Cancer Syndromes and other Cancers |
|
Biopsy Source
|
Peripheral vein
|
|
Cell Type
|
B-Lymphocyte
|
|
Tissue Type
|
Blood
|
|
Transformant
|
Epstein-Barr Virus
|
|
Sample Source
|
LCL from B-Lymphocyte
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Clinical summary/Case history
|
|
ISCN
|
46,XY{96}/46,XY,i(18)(qter>q10::q10> qter){4}
|
|
Species
|
Homo sapiens
|
|
Common Name
|
Human
|
|
Remarks
|
|
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
| |
| GENE MAPPING & DOSAGE STUDIES - CHROMOSOME 11 |
Gessler and Bruns (Genomics 5:43-55,1989) employed DNA from this cell culture to construct a long range restriction map of part of the short arm of chromosome 11 including the WAGR region using pulsed-field gel electrophoresis and a number of infrequently cutting restriction enzymes. |
| |
| Remarks |
Bilateral Wilms tumor; history of scrotal hypospadias, chordee, and hydrocoele; neoplasm showed extensive mesenchymal differentiation including sheets of skeletal and smooth muscle, island of cartilage, and islands of osteoid; 46,XY {96%} |
| Tang Z, Berlin DS, Toji L, Toruner GA, Beiswanger C, Kulkarni S, Martin CL, Emanuel BS, Christman M, Gerry NP, A dynamic database of microarray-characterized cell lines with various cytogenetic and genomic backgrounds G3 (Bethesda, Md)3:1143-9 2013 |
| PubMed ID: 23665875 |
| |
| Gessler M, Bruns GA, A physical map around the WAGR complex on the short arm of chromosome 11. Genomics5:43-55 1989 |
| PubMed ID: 2570029 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
|
|