GM06419
Fibroblast from Skin, Unspecified
Description:
RETINOBLASTOMA; RB1 OSTEOSARCOMA, RETINOBLASTOMA-RELATED, INCLUDED
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Heritable Diseases
Hereditary Cancers |
| Class |
Ophthalmologic Disorders |
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Biopsy Source
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Unspecified
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Unspecified
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Race
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White
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Ethnicity
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PERSIAN
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Family Member
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2
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Relation to Proband
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son
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XY
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
2 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Remarks |
Persian; bilateral stage IV retinoblastoma; diagnosed at age 8 mos; skin fibroblast culture; 46,XY; similarly affected father |
| Azzam EI, De Toledo SM, Spitz DR, Little JB, Oxidative metabolism modulates signal transduction and micronucleus formation in
bystander cells from alpha-particle-irradiated normal human fibroblast cultures. Cancer Res62(19):5436-42 2002 |
| PubMed ID: 12359750 |
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| Azzam EI, de Toledo SM, Waker AJ, Little JB, High and low fluences of alpha-particles induce a G1 checkpoint in human diploid
fibroblasts. Cancer Res60(10):2623-31 2000 |
| PubMed ID: 10825133 |
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| de Toledo SM, Azzam EI, Dahlberg WK, Gooding TB, Little JB, ATM complexes with HDM2 and promotes its rapid phosphorylation in a p53-independent manner in normal and tumor human cells exposed to ionizing radiation. Oncogene19(54):6185-93 2000 |
| PubMed ID: 11175332 |
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| Kumar S, Talis AL, Howley PM, Identification of HHR23A as a substrate for E6-associated protein-mediated ubiquitination. J Biol Chem274:18785-92 1999 |
| PubMed ID: 10373495 |
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| Sanford KK, Parshad R, Price FM, Tarone RE, Benedict WF, Cytogenetic responses to G2 phase x-irradiation of cells from retinoblastoma patients. Cancer Genet Cytogenet88:43-8 1996 |
| PubMed ID: 8630978 |
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| Li CY, Nagasawa H, Dahlberg WK, Little JB, Diminished capacity for p53 in mediating a radiation-induced G1 arrest in established human tumor cell lines. Oncogene11:1885-92 1995 |
| PubMed ID: 7478618 |
| Passage Frozen |
2 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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