GM04680
LCL from B-Lymphocyte
Description:
LEUKEMIA, CHRONIC MYELOID; CML
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Heritable Diseases |
| Class |
Heritable Cancer Syndromes and other Cancers |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Sample Source
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LCL from B-Lymphocyte
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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92,XXXX[25]/46,XX[50]
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Line IM9; G6PD type B; possesses surface receptors for human growth hormone, insulin, and calcitonin; synthesizes IgG; negative for reverse transcriptase; 60% 46,XX/40% 92,XXXX with random chromosome loss/gain |
| Caballero M, Ge T, Rebelo AR, Seo S, Kim S, Brooks K, Zuccaro M, Kanagaraj R, Vershkov D, Kim D, Smogorzewska A, Smolka M, Benvenisty N, West SC, Egli D, Mace EM, Koren A, Comprehensive analysis of DNA replication timing across 184 cell lines suggests a role for MCM10 in replication timing regulation Human molecular genetics: 2021 |
| PubMed ID: 35394024 |
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| Martins IJ, Hone E, Chi C, Seydel U, Martins RN, Redgrave TG, Relative roles of LDLr and LRP in the metabolism of chylomicron remnants in genetically manipulated mice. J Lipid Res41:205-13 2000 |
| PubMed ID: 10681404 |
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| Fantus IG, Saviolakis GA, Hedo JA, Gorden P, Mechanism of glucocorticoid-induced increase in insulin receptors of cultured human lymphocytes. J Biol Chem257:8277-83 1982 |
| PubMed ID: 7045118 |
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| Iwamoto Y, Maddux B, Goldfine ID, Chloroquine stimulation of insulin binding to IM-9 lymphocytes: evidence for action at a nonlysosomal site. Biochem Biophys Res Commun103:863-71 1981 |
| PubMed ID: 7036998 |
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| Van Obberghen E, De Meyts P, Roth J, Cell surface receptors for insulin and human growth hormone. Effect of microtubule and microfilament modifiers. J Biol Chem251:6844-51 1976 |
| PubMed ID: 185222 |
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| Gavin JR 3d, Roth J, Neville DM Jr, Meyts P de, Buell DN, Insulin-dependent regulation of insulin receptor concentrations: a direct demonstration in cell culture. Proc Natl Acad Sci U S A71:84-8 1974 |
| PubMed ID: 4359334 |
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| Lesniak MA, Gorden P, Roth J, Gavin JR 3d, Binding of 125I-human growth hormone to specific receptors in human cultured lymphocytes. Characterization of the interaction and a sensitive radioreceptor assay. J Biol Chem249:1661-7 1974 |
| PubMed ID: 4361817 |
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| Fahey JL, Buell DN, Sox HC, Proliferation and differentiation of lymphoid cells: studies with human lymphoid cell lines and immunoglobulin synthesis. Ann N Y Acad Sci190:221-34 1972 |
| PubMed ID: 5290016 |
| Split Ratio |
1:6 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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