GM03098
LCL from B-Lymphocyte
Description:
HLA HISTOCOMPATIBILITY TYPE: 142800 THROUGH 142860 & 142880
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Heritable Diseases |
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Biopsy Source
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Peripheral vein
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Cell Type
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B-Lymphocyte
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Tissue Type
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Blood
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Transformant
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Epstein-Barr Virus
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Sample Source
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LCL from B-Lymphocyte
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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46,XX
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| GENE MAPPING & DOSAGE STUDIES - Y CHROMOSOME |
PCR analysis of DNA from this cell culture gave a negative result with a primer for Yq11, DYS227. |
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| HLA TYPING |
HLA type; A:2,2; B:17,17; DR:3,3 |
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| Remarks |
HLA Type; A:2,2;B:17,17;DR:3,3 line WT49; 46,XX; 10% of cells show random chromosome loss/gain; consanguineous parents |
| Jameson J, Cruz J, Ennis FA, Human cytotoxic T-lymphocyte repertoire to influenza A viruses. J Virol72(11):8682-9 1998 |
| PubMed ID: 9765409 |
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| Mathew A, Kurane I, Green S, Stephens HA, Vaughn DW, Kalayanarooj S, Suntayakorn S, Chandanayingyong D, Ennis FA, Rothman AL, Predominance of HLA-restricted cytotoxic T-lymphocyte responses to serotype-cross-reactive epitopes on nonstructural proteins following natural secondary dengue virus infection. J Virol72(5):3999-4004 1998 |
| PubMed ID: 9557687 |
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| Woulfe SL, Bono CP, Zacheis ML, Welply JK, Kirschmann DA, Baudino TA, Wang Y, Stone DA, Hanson GJ, Vuletich JL, Bedell LJ, Schwartz BD, Howard SC, A peptidomimetic that specifically inhibits human leukocyte antigen DRB1*0401-restricted T cell proliferation. J Pharmacol Exp Ther281:663-9 1997 |
| PubMed ID: 9152371 |
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| Woulfe SL, Bono CP, Zacheis ML, Kirschmann DA, Baudino TA, Swearingen C, Karr RW, Schwartz BD, Negatively charged residues interacting with the p4 pocket confer binding specificity to DRB1*0401. Arthritis Rheum38:1744-53 1995 |
| PubMed ID: 8849346 |
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| Rommens JM, Zengerling S, Burns J, Melmer G, Kerem BS, Plavsic N, Zsiga M, Kennedy D, Markiewicz D, Rozmahel R, et al, Identification and regional localization of DNA markers on chromosome 7 for the cloning of the cystic fibrosis gene. Am J Hum Genet43:645-63 1988 |
| PubMed ID: 2903665 |
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| Zengerling S, Tsui LC, Grzeschik KH, Olek K, Riordan JR, Buchwald M, Mapping of DNA markers linked to the cystic fibrosis locus on the long arm of chromosome 7 [published erratum appears in Am J Hum Genet 1987 Aug;41(2):330] Am J Hum Genet40:228-36 1987 |
| PubMed ID: 3472464 |
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| Walker LE, Ketler TA, Houghten RA, Schulz G, Chersi A, Reisfeld RA, Human major histocompatibility complex class I antigens: residues 61-83 of the HLA-B7 heavy chain specify an alloreactive site. Proc Natl Acad Sci U S A82:539-42 1985 |
| PubMed ID: 3881768 |
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| Brodsky FM, A matrix approach to human class II histocompatibility antigens: reactions of four monoclonal antibodies with the products of nine haplotypes. Immunogenetics19:179-194 1984 |
| PubMed ID: 6200433 |
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| Howell DN, Kostyu DD, Ting JP, Cresswell P, Expression of class I histocompatibility antigens on human T-B lymphoblast hybrids. Somat Cell Mol Genet10:217-24 1984 |
| PubMed ID: 6609440 |
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| Engelhard VH, Benjamin C, Xenogeneic cytotoxic T-cell clones recognize alloantigenic determinants on HLA-A2. Immunogenetics18:461-473 1983 |
| PubMed ID: 6196283 |
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| Goyert SM, Silver J, Further characterization of HLA-DS molecules: implications for studies assessing the role of human Ia molecules in cell interactions and disease susceptibility. Proc Natl Acad Sci U S A80:5719-23 1983 |
| PubMed ID: 6193521 |
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| Shipp MA, Schwartz BD, Kannapell CC, Griffith RC, Scott MG, Ahmed P, Davie JM, Nahm MH, A unique DR-related B cell differentiation antigen. J Immunol131:2458-67 1983 |
| PubMed ID: 6415171 |
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| Ways JP, Parham P, The binding of monoclonal antibodies to cell-surface molecules. A quantitative analysis with immunoglobulin G against two alloantigenic determinants of the human transplantation antigen HLA-A2. Biochem J216:423-32 1983 |
| PubMed ID: 6197968 |
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| Quaranta V, Walker LE, Ruberto G, Pellegrino MA, Ferrone S, The free and the beta 2-microglobulin-associated heavy chains of HLA-A, B alloantigens share the antigenic determinant recognized by the monoclonal antibody Q1/28. Immunogenetics13:285-95 1981 |
| PubMed ID: 6168579 |
| dbSNP |
dbSNP ID: 10599 |
| Gene Cards |
HLA-A |
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HLA-DPA1 |
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HLA-DRA |
| Gene Ontology |
GO:0005887 integral to plasma membrane |
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GO:0006955 immune response |
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GO:0019883 antigen presentation, endogenous antigen |
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GO:0019884 antigen presentation, exogenous antigen |
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GO:0019885 antigen processing, endogenous antigen via MHC class I |
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GO:0019886 antigen processing, exogenous antigen via MHC class II |
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GO:0030106 MHC class I receptor activity |
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GO:0045012 MHC class II receptor activity |
| NCBI Gene |
Gene ID:3105 |
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Gene ID:3113 |
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Gene ID:3122 |
| NCBI GTR |
142800 MAJOR HISTOCOMPATIBILITY COMPLEX, CLASS I, A; HLA-A |
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142860 MAJOR HISTOCOMPATIBILITY COMPLEX, CLASS II, DR ALPHA; HLA-DRA |
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142880 MAJOR HISTOCOMPATIBILITY COMPLEX, CLASS II, DP ALPHA-1; HLA-DPA1 |
| OMIM |
142800 MAJOR HISTOCOMPATIBILITY COMPLEX, CLASS I, A; HLA-A |
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142860 MAJOR HISTOCOMPATIBILITY COMPLEX, CLASS II, DR ALPHA; HLA-DRA |
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142880 MAJOR HISTOCOMPATIBILITY COMPLEX, CLASS II, DP ALPHA-1; HLA-DPA1 |
| Omim Description |
HLA-A HISTOCOMPATIBILITY TYPE |
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MAJOR HISTOCOMPATIBILITY COMPLEX, CLASS I, A; HLA-A |
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HLA-DR HISTOCOMPATIBILITY TYPE; HLA-DRA |
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MAJOR HISTOCOMPATIBILITY COMPLEX II, DR ALPHA; HLA-DRA1 |
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HLA-SB HISTOCOMPATIBILITY TYPE; HLASB; HLADP |
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MAJOR HISTOCOMPATIBILITY COMPLEX, CLASS II, DP ALPHA-1; HLA-DPA1 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Roswell Park Memorial Institute Medium 1640 with 2mM L-glutamine or equivalent |
| Serum |
20% fetal bovine serum Not Inactivated |
| Substrate |
None specified |
| Subcultivation Method |
dilution - add fresh medium |
| Supplement |
- |
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