GM00638
Fibroblast from Skin, Unspecified
Description:
GALACTOSEMIA
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Heritable Diseases |
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Biopsy Source
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Unspecified
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Simian Virus 40
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Sample Source
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Fibroblast from Skin, Unspecified
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Race
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White
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
42 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
SV40 transformed GM00054; Gal-1-P uridyl transferase absent post-crisis, G6PD type B; T-antigen positive; see GM01907 Fibroblast and GM02795A Lymphoid; hypodiploid, unstable karyotype with random rearrangements and 3 marker chromosomes |
| Lai K, Langley SD, Khwaja FW, Schmitt EW, Elsas LJ, GALT deficiency causes UDP-hexose deficit in human galactosemic cells Glycobiology13:285-94 2003 |
| PubMed ID: 12626383 |
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| Prince PR, Ogburn CE, Moser MJ, Emond MJ, Martin GM, Monnat RJ Jr, Cell fusion corrects the 4-nitroquinoline 1-oxide sensitivity of Werner syndrome fibroblast cell lines. Hum Genet105:132-8 1999 |
| PubMed ID: 10480367 |
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| Reichardt JK, Molecular analysis of 11 galactosemia patients. Nucleic Acids Res19:7049-52 1991 |
| PubMed ID: 1766867 |
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| Babich MA, Day RS 3d, Synergistic killing of virus-transformed human cells with interferon and N-methyl-N'-nitro-N-nitrosoguanidine. Carcinogenesis10:265-8 1989 |
| PubMed ID: 2463881 |
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| Baldwin GC, Gasson JC, Kaufman SE, Quan SG, Williams RE, Avalos BR, Gazdar AF, Golde DW, DiPersio JF, Nonhematopoietic tumor cells express functional GM-CSF receptors. Blood73:1033-7 1989 |
| PubMed ID: 2537665 |
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| Choi KH, Tevethia SS, Shin S, Tumor formation by SV40-transformed human cells in nude mice: the role of SV40 T antigens. Cytogenet Cell Genet36:633-40 1983 |
| PubMed ID: 6319085 |
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| Day RS 3d, Ziolkowski CH, Scudiero DA, Meyer SA, Lubiniecki AS, Girardi AJ, Galloway SM, Bynum GD, Defective repair of alkylated DNA by human tumour and SV40-transformed human cell strains. Nature288:724-7 1980 |
| PubMed ID: 6256643 |
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| Farber RA, Davidson RL, Maintenance of replication patterns in human-mouse hybrids retaining only one human chromosome. Somatic Cell Genet5:519-28 1979 |
| PubMed ID: 227124 |
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| Greene AE, Manduka M, Coriell LL, SV40 virus transformation of genetic mutant cell cultures. Monogr Hum Genet9:248-52 1978 |
| PubMed ID: 215903 |
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| Croce CM, Assignment of the integration site for simian virus 40 to chromosome 17 in GM54VA, a human cell line transformed by simian virus 40. Proc Natl Acad Sci U S A74:315-8 1977 |
| PubMed ID: 189310 |
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| Tan YH, Berthold W, A mechanism for the induction and regulation of human fibroblastoid interferon genetic expression. J Gen Virol34:401-11 1977 |
| PubMed ID: 856944 |
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| Tan YH, Greene AE, Subregional localization of the gene(s) governing the human interferon induced antiviral state in man. J Gen Virol32:153-5 1976 |
| PubMed ID: 182912 |
| Passage Frozen |
42 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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