AG21201
Fibroblast from Skin, Ear
Description:
RATTUS
ORDER: RODENTIA
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals |
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Biopsy Source
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Ear
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Ear
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Rattus norvegicus
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Common Name
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brown or Norway rat
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Remarks
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| Passage Frozen |
2 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Skin fibroblast culture made from explants of ear skin from a calorically restricted Fisher F344 rat. Caloric Restriction (CR) is initiated at 14 weeks of age at 10% restriction, increased to 25% restriction at 15 weeks, and to 40% restriction at 16 weeks where it is maintained throughout the life of the animal. CR rats are fed a fortified food to ensure their micronutrient intake is maintained at optimal levels. Survival curves for rats and mice under this CR protocol have been published (PMID 10619312). |
| Turturro A, Witt WW, Lewis S, Hass BS, Lipman RD, Hart RW, Growth curves and survival characteristics of the animals used in the Biomarkers of Aging Program The journals of gerontology Series A, Biological sciences and medical sciences54:B492-501 2000 |
| PubMed ID: 10619312 |
| Cumulative PDL at Freeze |
5.38 |
| Passage Frozen |
2 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Ham's F12 Medium/Dulbecco Modified Eagles Medium, 1:1 mixture with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Supplement |
- |
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