AG20592
Fibroblast from Skin, Unspecified
Description:
GALAGO SENEGALENSIS
ORDER: PRIMATES
Repository
|
NIA Aging Cell Culture Repository
|
Subcollection |
Nonhuman Primates Animals |
Biopsy Source
|
Unspecified
|
Cell Type
|
Fibroblast
|
Tissue Type
|
Skin
|
Transformant
|
Untransformed
|
Sample Source
|
Fibroblast from Skin, Unspecified
|
Relation to Proband
|
proband
|
Confirmation
|
Clinical summary/Case history
|
ISCN
|
38,XY
|
Species
|
Galago senegalensis
|
Common Name
|
Senegal bush baby
|
Remarks
|
|
Passage Frozen |
7 |
|
IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
|
Remarks |
Senegal bush baby |
Passage Frozen |
7 |
Split Ratio |
1:3 |
Temperature |
37 C |
Percent CO2 |
5% |
Medium |
MEM (Eagle) Alpha Modification with nucleosides/Dulbecco Modified Eagles Medium (high glucose), 1:1 mixture with 2mM L-glutamine or equivalent |
Serum |
15% fetal bovine serum Not inactivated |
Substrate |
None specified |
Subcultivation Method |
trypsin-EDTA |
Supplement |
Sodium Pyruvate 100mM |
|
|