AG12363
Fibroblast from Lung, Lung
Description:
SUS
ORDER: CETARTIODACTYLA
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals |
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Biopsy Source
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Lung
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Cell Type
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Fibroblast
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Tissue Type
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Lung
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Lung, Lung
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Ethnicity
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YORKSHIRE
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Sus scrofa
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Common Name
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pig
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Remarks
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| PDL at Freeze |
13 |
| Passage Frozen |
5 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Yorkshire breed. The lung biopsy was taken immediately post-mortem. The culture was initiated using explants of minced tissue. Cell morphology is fibroblast-like. The karyotype is 38,XX with 2% of the cells examined showing random chromosome gain. Other cultures from this pig are AG12362, AG12372, and AG12417. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Cumulative PDL at Freeze |
13 |
| Passage Frozen |
5 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Ham's F12 with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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