AG12362
Fibroblast from Skin, Skin
Description:
SUS
ORDER: CETARTIODACTYLA
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals |
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Biopsy Source
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Skin
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Skin
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Ethnicity
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YORKSHIRE
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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38,XX
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Species
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Sus scrofa
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Common Name
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pig
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Remarks
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| PDL at Senescence |
19 |
| PDL at Freeze |
6 |
| Passage Frozen |
3 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Yorkshire breed. The skin biopsy was taken immediately post-mortem. The culture was initiated using explants of minced tissue. Cell morphology is fibroblast-like. The karyotype is 38,XX; normal diploid female. Other cultures from this pig are AG12363, AG12372, and AG12417. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| PDL at Senescence |
19 |
| Cumulative PDL at Freeze |
6 |
| Passage Frozen |
3 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
Endotoxin-Free Ham's F12 Medium with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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