AG12119
Fibroblast from Skin, Skin
Description:
SUS
ORDER: CETARTIODACTYLA
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals |
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Biopsy Source
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Skin
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Skin
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Ethnicity
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YORKSHIRE
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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38,XX
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Species
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Sus scrofa
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Common Name
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pig
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Remarks
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| PDL at Freeze |
17 |
| Passage Frozen |
5 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Yorkshire breed. The skin biopsy was taken immediately post-mortem on 3/12/92. The culture was initiated using explants of minced tissue. Cell morphology is fibroblast-like. The karyotype is 38,XX with 12% of the cells examined showing random chromosome loss/gain and 2% showing random chromosomal aberrations. Other cultures from this pig are AG12077 and AG12120. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Hofmann W, Schubert D, LaBonte J, Munson L, Gibson S, Scammell J, Ferrigno P, Sodroski J, Species-specific, postentry barriers to primate immunodeficiency virus infection. J Virol73:10020-8 1999 |
| PubMed ID: 10559316 |
| Cumulative PDL at Freeze |
17 |
| Passage Frozen |
5 |
| Split Ratio |
1:7 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Ham's F12 with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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