AG08473
Smooth muscle from Aorta, thoracic
Description:
SUS
ORDER: CETARTIODACTYLA
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals |
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Biopsy Source
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Aorta, thoracic
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Cell Type
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Smooth muscle
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Tissue Type
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Artery
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Transformant
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Untransformed
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Sample Source
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Smooth muscle from Aorta, thoracic
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Sus scrofa
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Common Name
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pig
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Remarks
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| PDL at Senescence |
145 |
| PDL at Freeze |
11 |
| Passage Frozen |
5 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Genbank |
COX1 |
| Remarks |
Large white domestic breed. The age of the donor is estimated. The thoracic aorta was removed immediately post-mortem on 10/15/85. Strips of the medial layer were removed, minced, and used as explants to initiate the culture. Cell morphology is fibroblast-like. The karyotype is 38,XX; normal diploid female. The culture tested positive for alpha-smooth muscle actin and negative for the endothelial cell-specific von Willebrand factor (immunofluorescence assays). For lifespan determination this cell line was cultured on gelatin-coated flasks and the medium was supplemented with crude ECGF (0.11mg/ml) and heparin (0.1mg/ml). An endothelial cell culture from this pig is AG08472A. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Motterlini R, Kerger H, Green CJ, Winslow RM, Intaglietta M, Depression of endothelial and smooth muscle cell oxygen consumption by endotoxin. Am J Physiol275:H776-82 1998 |
| PubMed ID: 9724279 |
| PDL at Senescence |
145 |
| Cumulative PDL at Freeze |
11 |
| Passage Frozen |
5 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
10% fetal bovine serum Not inactivated |
| Substrate |
Gelatin |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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