AG08472
Endothelial from Aorta, thoracic
Description:
SUS
ORDER: CETARTIODACTYLA
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals |
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Biopsy Source
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Aorta, thoracic
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Cell Type
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Endothelial
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Tissue Type
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Artery
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Transformant
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Untransformed
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Sample Source
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Endothelial from Aorta, thoracic
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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38,XX
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Species
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Sus scrofa
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Common Name
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pig
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Remarks
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| PDL at Senescence |
115 |
| PDL at Freeze |
15 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Remarks |
Large white domestic breed. The age of the donor is estimated. The thoracic aorta was removed immediately post-mortem on 10/15/85. The culture was initiated using cells released from the lumen of the vessel by collagenase digestion. The cell morphology is polygonal. The karyotype is 38,XX with 4% of the cells examined showing random chromosome loss and 2% showing random chromosomal aberrations. The culture tested positive for the endothelial cell-specific von Willebrand factor and negative for alpha-smooth muscle actin (immunofluorescence assays). The culture was grown on gelatin coated flasks and the medium was supplemented with ECGS (0.02 mg/ml) and heparin (0.05 mg/ml). Culture medium: AMMC 15U. with supplements. For lifespan determination this cell line was cultured in Eagle's medium with supplements and 20% inactivated fetal bovine serum. A smooth muscle cell culture from this pig is AG08473A. This cell line will only be shipped frozen. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Motterlini R, Kerger H, Green CJ, Winslow RM, Intaglietta M, Depression of endothelial and smooth muscle cell oxygen consumption by endotoxin. Am J Physiol275:H776-82 1998 |
| PubMed ID: 9724279 |
| PDL at Senescence |
115 |
| Cumulative PDL at Freeze |
15 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
MEM (Eagle) Alpha Modification with nucleosides/MCDB110, 1:1 mixture with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
Gelatin |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Endothelial Cell Growth Supplement 0.02 mg/ml |
| Supplement |
Heparin 0.05 mg/ml |
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