AG08421
Endothelial from Aorta, thoracic
Description:
EQUUS
ORDER: PERISSODACTYLA
|
Repository
|
NIA Aging Cell Culture Repository
|
| Subcollection |
Animals |
|
Biopsy Source
|
Aorta, thoracic
|
|
Cell Type
|
Endothelial
|
|
Tissue Type
|
Artery
|
|
Transformant
|
Untransformed
|
|
Sample Source
|
Endothelial from Aorta, thoracic
|
|
Family Member
|
1
|
|
Relation to Proband
|
proband
|
|
Confirmation
|
Clinical summary/Case history
|
|
Species
|
Equus caballus
|
|
Common Name
|
horse
|
|
Remarks
|
|
| PDL at Senescence |
22 |
| PDL at Freeze |
7 |
| Passage Frozen |
2 |
| |
| Remarks |
Thoroughbred horse. The thoracic aorta was removed 1 hour post-mortem on 9/18/85. The culture was initiated using cells released from the lumen of the vessel by collagenase digestion. Cell morphology is polygonal. The cells were grown on gelatin-coated flasks in medium supplemented with horse plasma-derived serum and heparin (0.1mg/ml). The culture was frozen in medium with fetal bovine serum, ECGS (0.02 mg/ml) and heparin (0.05 mg/ml). The culture tested positive forthe endothelial cell-specific von Willebrand factor and negative for alpha-smooth muscle actin (immunofluorescence assays). For lifespan determination this cell line was cultured in Ham's F12 medium and MCDB104 medium in a 1/1 mixture with 10% inactivated fetal bovine serum and supplements (0.04-0.06mg/ml crude ECGF and 0.05mg/ml heparin). Culture medium: AMMC 20U with supplements. Other cultures from this horse are AG08419, AG08420, and AG08422. This cell line will only be shipped frozen. |
| PDL at Senescence |
22 |
| Cumulative PDL at Freeze |
7 |
| Passage Frozen |
2 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
MEM (Eagle) Alpha Modification with nucleosides/MCDB110, 1:1 mixture with 2mM L-glutamine or equivalent |
| Serum |
20% fetal bovine serum Not inactivated |
| Substrate |
Gelatin |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Endothelial Cell Growth Supplement 0.02 mg/ml |
| Supplement |
Heparin 0.05 mg/ml |
|
|