AG08259
Fibroblast from Skin, Skin
Description:
ALZHEIMER DISEASE; AD
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Alzheimer's Disease
Alzheimer Disease With No Family History |
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Biopsy Source
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Skin
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Skin
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Race
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Black/African American
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| PDL at Freeze |
4.52 |
| Passage Frozen |
4 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by LINE assay |
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| Remarks |
The donor had a history of progressive dementia since age 87 years. Autopsy of brain revealed numerous neurofibrillary tangles and plaques. The skin biopsy was taken post-mortem on 6/17/85. The cell morphology is fibroblast-like. Karyotypeis 46,XY; normal diploid male. Same subject as AG08258 (lymph) and AG27711 (stem cell). The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Hanna R, Flamier A, Barabino A, Bernier G, G-quadruplexes originating from evolutionary conserved L1 elements interfere with neuronal gene expression in Alzheimer's disease Nature communications12:1828 2019 |
| PubMed ID: 33758195 |
| Cumulative PDL at Freeze |
9.52 |
| Passage Frozen |
4 |
| Split Ratio |
1:2 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Supplement |
- |
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