AG08031
Smooth muscle from Aorta, thoracic
Description:
EQUUS
ORDER: PERISSODACTYLA
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals |
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Biopsy Source
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Aorta, thoracic
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Cell Type
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Smooth muscle
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Tissue Type
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Artery
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Transformant
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Untransformed
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Sample Source
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Smooth muscle from Aorta, thoracic
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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64,XX
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Species
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Equus caballus
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Common Name
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horse
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Remarks
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| PDL at Freeze |
13 |
| Passage Frozen |
3 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Remarks |
Standardbred horse. The thoracic aorta was removed 1 hour post-mortem on 2/27/85. Strips of the medial layer were minced, treated with collagenase and then elastase. Cells released by this treatment were used for the primary culture. Cell morphology is fibroblast-like and the cells form layered sheets at post-confluence. The karyotype is 64,XX; normal diploid female. The culture tested positive for alpha-smooth muscle actin and negative for the endothelial cell-specific von Willebrand factor (immunofluorescence assays). Other cultures from this horse are AG08029 and AG08030. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| Cumulative PDL at Freeze |
13 |
| Passage Frozen |
3 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
MEM (Eagle) with 2X conc. A.A. and VIT./MCDB110, 1:1 mixture with 2mM L-glutamine or equivalent |
| Serum |
20% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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