AG08030
Endothelial from Aorta, thoracic
Description:
EQUUS
ORDER: PERISSODACTYLA
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals |
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Biopsy Source
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Aorta, thoracic
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Cell Type
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Endothelial
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Tissue Type
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Artery
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Transformant
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Untransformed
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Sample Source
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Endothelial from Aorta, thoracic
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Family Member
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1
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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64,XX
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Species
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Equus caballus
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Common Name
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horse
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Remarks
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| PDL at Senescence |
23 |
| PDL at Freeze |
11 |
| Passage Frozen |
2 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Chromosome Analysis |
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| Remarks |
(Standardbred horse) The thoracic aorta was removed 1 hour post-mortem on 2/27/85. The culture was initiated using cellsreleased from the lumen of the vessel by collagenase digestion. The cell morphology is polygonal. The culture was grown on gelatin-coated flasks, and the medium was supplemented with ECGS (0.02 mg/ml) and heparin (0.05 mg/ml). The karyotype is 64,XX; normal diploid female. Culture submitted by F Angelosanto (#0477). Culture medium: AMMC 20U (with supplements). Other cultures from this horse are AG08029 and AG08031. This cell line will only be shipped frozen. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| PDL at Senescence |
23 |
| Cumulative PDL at Freeze |
11 |
| Passage Frozen |
2 |
| Split Ratio |
1:5 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
MEM (Eagle) Alpha Modification with nucleosides/MCDB110, 1:1 mixture with 2mM L-glutamine or equivalent |
| Serum |
20% fetal bovine serum Not inactivated |
| Substrate |
Gelatin |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
Endothelial Cell Growth Supplement 0.02 mg/ml |
| Supplement |
Heparin 0.05 mg/ml |
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