AG05253
Fibroblast from Skin, Thigh
Description:
PAN PANISCUS
ORDER: PRIMATES
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Nonhuman Primates
Animals |
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Biopsy Source
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Thigh
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Thigh
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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48,XY
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Species
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Pan paniscus
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Common Name
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bonobo
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Remarks
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| PDL at Freeze |
3 |
| Passage Frozen |
13 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase, Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Genbank |
Lipoprotein Lp(a) |
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| Genbank |
Apolipoprotein B |
| Babcock M, Yatsenko S, Stankiewicz P, Lupski JR, Morrow BE, AT-rich repeats associated with chromosome 22q112 rearrangement disorders shape human genome architecture on Yq12 Genome research17:451-60 2007 |
| PubMed ID: 17284672 |
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| Callinan PA, Wang J, Herke SW, Garber RK, Liang P, Batzer MA, Alu retrotransposition-mediated deletion. J Mol Biol348(4):791-800 2005 |
| PubMed ID: 15843013 |
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| Han K, Sen SK, Wang J, Callinan PA, Lee J, Cordaux R, Liang P, Batzer MA, Genomic rearrangements by LINE-1 insertion-mediated deletion in the human and chimpanzee lineages. Nucleic Acids Res33(13):4040-52 2005 |
| PubMed ID: 16034026 |
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| Ho HJ, Ray DA, Salem AH, Myers JS, Batzer MA, Straightening out the LINEs: LINE-1 orthologous loci. Genomics85(2):201-7 2005 |
| PubMed ID: 15676278 |
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| Ray DA, Xing J, Hedges DJ, Hall MA, Laborde ME, Anders BA, White BR, Stoilova N, Fowlkes JD, Landry KE, Chemnick LG, Ryder OA, Batzer MA, Alu insertion loci and platyrrhine primate phylogeny. Mol Phylogenet Evol35(1):117-26 2005 |
| PubMed ID: 15737586 |
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| Salem AH, Ray DA, Hedges DJ, Jurka J, Batzer MA, Analysis of the human Alu Ye lineage. BMC Evol Biol5(1):18 2005 |
| PubMed ID: 15725352 |
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| Fortna A, Kim Y, MacLaren E, Marshall K, Hahn G, Meltesen L, Brenton M, Hink R, Burgers S, Hernandez-Boussard T, Karimpour-Fard A, Glueck D, McGavran L, Berry R, Pollack J, Sikela JM, Lineage-specific gene duplication and loss in human and great ape evolution. PLoS Biol2(7):E207 2004 |
| PubMed ID: 15252450 |
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| Hedges DJ, Callinan PA, Cordaux R, Xing J, Barnes E, Batzer MA, Differential alu mobilization and polymorphism among the human and chimpanzee lineages. Genome Res14(6):1068-75 2004 |
| PubMed ID: 15173113 |
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| Otieno AC, Carter AB, Hedges DJ, Walker JA, Ray DA, Garber RK, Anders BA, Stoilova N, Laborde ME, Fowlkes JD, Huang CH, Perodeau B, Batzer MA, Analysis of the human Alu Ya-lineage. J Mol Biol342(1):109-18 2004 |
| PubMed ID: 15313610 |
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| Salem AH, Kilroy GE, Watkins WS, Jorde LB, Batzer MA, Recently integrated Alu elements and human genomic diversity. Mol Biol Evol20(8):1349-61 2003 |
| PubMed ID: 12777511 |
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| Morrish TA, Gilbert N, Myers JS, Vincent BJ, Stamato TD, Taccioli GE, Batzer MA,
Moran JV, DNA repair mediated by endonuclease-independent LINE-1 retrotransposition. Nat Genet31(2):159-65 2002 |
| PubMed ID: 12006980 |
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| Myers JS, Vincent BJ, Udall H, Watkins WS, Morrish TA, Kilroy GE, Swergold GD, Henke J, Henke L, Moran JV, Jorde LB, Batzer MA, A comprehensive analysis of recently integrated human Ta L1 elements. Am J Hum Genet71(2):312-26 2002 |
| PubMed ID: 12070800 |
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| Ovchinnikov I, Rubin A, Swergold GD, Tracing the LINEs of human evolution. Proc Natl Acad Sci U S A99(16):10522-7 2002 |
| PubMed ID: 12138175 |
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| Steinert S, White DM, Zou Y, Shay JW, Wright WE, Telomere biology and cellular aging in nonhuman primate cells. Exp Cell Res272(2):146-52 2002 |
| PubMed ID: 11777339 |
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| Shaikh TH, Kurahashi H, Emanuel BS, Evolutionarily conserved low copy repeats (LCRs) in 22q11 mediate deletions, duplications, translocations, and genomic instability: an update and literature review. Genet Med3(1):6-13 2001 |
| PubMed ID: 11339380 |
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| Barrientos A, Kenyon L, Moraes CT, Human xenomitochondrial cybrids. Cellular models of mitochondrial complex I deficiency. J Biol Chem273(23):14210-7 1998 |
| PubMed ID: 9603924 |
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| Kenyon L,Moraes C, Expanding the functional human mitochondrial DNA database by the establishment of primate xenomitochondrial cybrids. Proc Natl Acad Sci U S A94:9131-5 1997 |
| PubMed ID: 9256447 |
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| Schempp W, Weber B, Muller G, Mammalian sex-chromosome evolution: a conserved homoeologous segment on the X and Y chromosomes in primates. Cytogenet Cell Genet50:201-5 1989 |
| PubMed ID: 2805817 |
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| Nakamura KD, Hart RW, Comparison of proto oncogene expression in seven primate fibroblast cultures. Mech Ageing Dev39:177-87 1987 |
| PubMed ID: 3626640 |
| Cumulative PDL at Freeze |
14 |
| Passage Frozen |
13 |
| Split Ratio |
1:2 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Medium |
MEM (Eagle) Alpha Modification with nucleosides with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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