AG05056
Epithelial from Eye
Description:
SUS
ORDER: CETARTIODACTYLA
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals |
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Biopsy Source
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Eye
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Cell Type
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Epithelial
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Tissue Type
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Lens
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Transformant
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Untransformed
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Sample Source
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Epithelial from Eye
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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Heteroploid; 100% chromosomally abnormal counts range from 32-142; karyotype compatible with pig
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Species
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Sus scrofa
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Common Name
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pig
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Remarks
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| PDL at Freeze |
21 |
| |
| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase Isoenzyme Electrophoresis and by Chromosome Analysis |
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| Genbank |
COX1 |
| Remarks |
The culture was initiated on 11/6/79 by plating a suspension of single cells obtained from the lens capsule of an adult pig of unknown age. Cell morphology is epithelial-like. The cell line is heteroploid with all cells examined showing chromosomal abnormalities. |
| Hofmann W, Schubert D, LaBonte J, Munson L, Gibson S, Scammell J, Ferrigno P, Sodroski J, Species-specific, postentry barriers to primate immunodeficiency virus infection. J Virol73:10020-8 1999 |
| PubMed ID: 10559316 |
| Cumulative PDL at Freeze |
21 |
| Split Ratio |
1:4 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Eagle's Minimum Essential Medium with Earle's salts and non-essential amino acids with 2mM L-glutamine or equivalent |
| Serum |
20% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Subcultivation Method |
trypsin-EDTA |
| Supplement |
- |
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