GM25430
iPSC from Fibroblast
Description:
APPARENTLY HEALTHY INDIVIDUAL
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Repository
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NIGMS Human Genetic Cell Repository
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| Subcollection |
Apparently healthy iPSCs
Apparently Healthy Collection |
| Protocols |
Protocol PDF |
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Biopsy Source
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Skin
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Cell Type
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Stem cell
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Cell Subtype
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Induced pluripotent stem cell
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Transformant
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Reprogrammed (Retroviral)
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Sample Source
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iPSC from Fibroblast
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Race
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White
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Ethnicity
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Not Hispanic/Latino
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Country of Origin
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USA
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Family Member
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1
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Family History
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N
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Relation to Proband
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proband
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ISCN
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46,XY[24].arr[hg19](1-22)x2,(X,Y)x1
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Species
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Homo sapiens
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Common Name
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Human
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Remarks
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| Passage Frozen |
26 |
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| Induced Pluripotent Stem Cell |
The frozen cell line submitted to the Repository was recovered and expanded. The expanded line was evaluated for viability surface antigen expression and alkaline phosphatase activity. Pluripotency was assessed via embryoid body (EB) formation and PluriTest. Steady-state mRNA expression patterns of undifferentiated iPSC and EBs were determined via real-time PCR. Characterization data are included in the Certificate of Analysis. |
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| Remarks |
Apparently healthy individual (J. Craig Venter); induced pluripotent stem cell line derived from retroviral reprogramming of fibroblast cells (skin, arm); parental fibroblast not in repository; see NS12911 for DNA; for more information, go to: https://catalog.coriell.org/1/HuRef. This iPSC line, CV-hiPSC-B or CVB, was submitted by Dr. Lawrence Goldstein (University of California San Diego). Researchers purchasing hiPSCs from the NIGMS Repository are responsible for any limited use label licenses (LULLs) applicable to the cell line purchased. The applicable LULL to this line is iPS Academia, Japan Inc.. |
| Lee H, Aylward AJ, Pearse RV, Hsieh YC, Augur ZM, Benoit CR, Chou V, Knupp A, Pan C, Goberdhan S, Duong DM, Seyfried NT, Bennett DA, Klein HU, De Jager PL, Menon V, Young JE, Young-Pearse TL, Cell-type-specific regulation of APOE levels in human neurons by the Alzheimer's disease risk gene SORL1 bioRxiv : the preprint server for biology: 2023 |
| PubMed ID: 36865313 |
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| Cho H, Abshire ET, Popp MW, Pröschel C, Schwartz JL, Yeo GW, Maquat LE, AKT constitutes a signal-promoted alternative exon-junction complex that regulates nonsense-mediated mRNA decay Molecular cell82:2779-2796.e10 2022 |
| PubMed ID: 35675814 |
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| Mahoney R, Ochoa Thomas E, Ramirez P, Miller HE, Beckmann A, Zuniga G, Dobrowolski R, Frost B, Pathogenic Tau Causes a Toxic Depletion of Nuclear Calcium Cell reports32:107900 2020 |
| PubMed ID: 32668249 |
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| Jain M, Olsen HE, Turner DJ, Stoddart D, Bulazel KV, Paten B, Haussler D, Willard HF, Akeson M, Miga KH, Linear assembly of a human centromere on the Y chromosome Nature biotechnology36:321-323 2017 |
| PubMed ID: 29553574 |
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| Woodruff G, Young JE, Martinez FJ, Buen F, Gore A, Kinaga J, Li Z, Yuan SH, Zhang K, Goldstein LS, The presenilin-1 ?E9 mutation results in reduced ?-secretase activity, but not total loss of PS1 function, in isogenic human stem cells Cell reports5:974-85 2013 |
| PubMed ID: 24239350 |
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| Gore A, Li Z, Fung HL, Young JE, Agarwal S, Antosiewicz-Bourget J, Canto I, Giorgetti A, Israel MA, Kiskinis E, Lee JH, Loh YH, Manos PD, Montserrat N, Panopoulos AD, Ruiz S, Wilbert ML, Yu J, Kirkness EF, Izpisua Belmonte JC, Rossi DJ, Thomson JA, Eggan K, Daley GQ, Goldstein LS, Zhang K, Somatic coding mutations in human induced pluripotent stem cells Nature471:63-7 2010 |
| PubMed ID: 21368825 |
| Passage Frozen |
26 |
| Split Ratio |
1:6 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
AMBIENT |
| Medium |
Ham's F12 Medium/Dulbecco Modified Eagles Medium, 1:1 mixture with 2mM L-glutamine or equivalent |
| Serum |
20% Knock-out Serum Replacement Not inactivated |
| Substrate |
Gelatin + Feeder Layer |
| Supplement |
Basic Fibroblast Growth Factor 10 ng/ml |
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