AG22874
Fibroblast from Skin, Ear
Description:
AMES DWARF MOUSE PROJECT
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Repository
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NIA Aging Cell Culture Repository
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| Subcollection |
Animals
Ames Dwarf Mice |
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Biopsy Source
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Ear
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Cell Type
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Fibroblast
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Tissue Type
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Skin
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Transformant
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Untransformed
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Sample Source
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Fibroblast from Skin, Ear
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Family Member
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1
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Family History
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N
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Relation to Proband
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proband
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Confirmation
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Clinical summary/Case history
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ISCN
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40~44,X~XY,+6,+10,+10,+15,+19[cp17]/40,XY[3]
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Species
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Mus musculus
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Common Name
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house mouse
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Remarks
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| PDL at Senescence |
31 |
| PDL at Freeze |
5.19 |
| Passage Frozen |
2 |
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| IDENTIFICATION OF SPECIES OF ORIGIN |
Species of Origin Confirmed by Nucleoside Phosphorylase,Glucose-6-Phosphate Dehydrogenase, and Lactate Dehydrogenase Isoenzyme Electrophoresis |
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| Remarks |
Ames dwarf mouse; this mutant is homozygous for a recessive mutation in the Prop1 gene [Prop1df/Prop1df]; skin fibroblast culture was established from minced ear tissue taken at time of euthanasia. Cells show abnormal karyotype. The legacy karyotype description shown in this Remark may not be representative of the current available product. |
| PDL at Senescence |
31 |
| Cumulative PDL at Freeze |
5.19 |
| Passage Frozen |
2 |
| Split Ratio |
1:3 |
| Temperature |
37 C |
| Percent CO2 |
5% |
| Percent O2 |
3% |
| Medium |
Ham's F12 Medium/Dulbecco Modified Eagles Medium, 1:1 mixture with 2mM L-glutamine or equivalent |
| Serum |
15% fetal bovine serum Not inactivated |
| Substrate |
None specified |
| Supplement |
- |
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